Hot spots in ag core-au shell nanoparticles potent for surface-enhanced Raman scattering studies of biomolecules

被引:161
作者
Kumar, G. V. Pavan
Shruthi, S.
Vibha, B.
Reddy, B. A. Ashok
Kundu, Tapas K.
Narayana, Chandrabhas [1 ]
机构
[1] Jawaharlal Nehru Ctr Adv Sci Res, Light Scattering Lab, Chem & Phys Mat Unit, Bangalore 560064, Karnataka, India
[2] Jawaharlal Nehru Ctr Adv Sci Res, Dis Lab, Mol Biol & Genet Unit, Bangalore 560064, Karnataka, India
关键词
D O I
10.1021/jp068253n
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The vicinity of metallic nanostructures that provides intense optical fields is termed as "hot spot". Any molecule in close proximity to these hot spots will give rise to an increased surface-enhanced Raman spectroscopic (SERS) signal. We have synthesized Ag core-Au shell (core-shell) nanoparticles (NP) with nanopores, which act as hot spots in the SERS measurements. We have demonstrated a large enhancement in SERS studies of various molecules using core-shell NP with hot spots, which is better than using silver nanoparticles. The core-shell NP with hot spots can be used for ultratrace analysis of important biomolecules such as histone acetyltranferase p300, a human transcriptional coactivator protein. The core-shell NP does not change the biomolecule's physical and chemical property upon adsorption, which makes it biocompatible. The core-shell NP carrying hot spots with high SERS enhancement would be ideally suited for in vivo studies of biological systems.
引用
收藏
页码:4388 / 4392
页数:5
相关论文
共 31 条
[1]   Surface-enhanced Raman scattering on nanoshells with tunable surface plasmon resonance [J].
Alvarez-Puebla, RA ;
Ross, DJ ;
Nazri, GA ;
Aroca, RF .
LANGMUIR, 2005, 21 (23) :10504-10508
[2]   Surface-enhanced resonance Raman spectroscopy signals from single myoglobin molecules [J].
Bizzarri, AR ;
Cannistraro, S .
APPLIED SPECTROSCOPY, 2002, 56 (12) :1531-1537
[3]   Near-field and far-field scattering by bimetallic nanoshell systems [J].
Bruzzone, S. ;
Malvaldi, M. ;
Arrighini, G. P. ;
Guidotti, C. .
JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (23) :11050-11054
[4]   SURFACE-ENHANCED RAMAN-SCATTERING OF ADENOSINE-TRIPHOSPHATE MOLECULES [J].
CHEN, TT ;
KUO, CS ;
CHOU, YC ;
LIANG, NT .
LANGMUIR, 1989, 5 (04) :887-891
[5]   SURFACE-ENHANCED RAMAN-SPECTROSCOPY OF BIOMOLECULES .1. WATER-SOLUBLE PROTEINS, DIPEPTIDES AND AMINO-ACIDS [J].
CHUMANOV, GD ;
EFREMOV, RG ;
NABIEV, IR .
JOURNAL OF RAMAN SPECTROSCOPY, 1990, 21 (01) :43-48
[6]   Synthesis of AgcoreAushell bimetallic nanoparticles for immunoassay based on surface-enhanced Raman spectroscopy [J].
Cui, Y ;
Ren, B ;
Yao, JL ;
Gu, RA ;
Tian, ZQ .
JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (09) :4002-4006
[7]  
Ermishov M, 2000, BIOPOLYMERS, V57, P272, DOI 10.1002/1097-0282(2000)57:5<272::AID-BIP40>3.0.CO
[8]  
2-V
[9]   Observation of dynamic oxygen release in hemoglobin using surface enhanced Raman scattering [J].
Etchegoin, P ;
Liem, H ;
Maher, RC ;
Cohen, LF ;
Brown, RJC ;
Milton, MJT ;
Gallop, JC .
CHEMICAL PHYSICS LETTERS, 2003, 367 (1-2) :223-229
[10]   Surface-enhanced Raman spectroscopy study of the interaction of the antitumoral drug emodin with human serum albumin [J].
Fabriciova, G ;
Sanchez-Cortes, S ;
Garcia-Ramos, JV ;
Miskovsky, P .
BIOPOLYMERS, 2004, 74 (1-2) :125-130