Lignin biosynthesis in poplar:: Genetic engineering and effects on kraft pulping

被引：7

作者：

Boerjan, W ^{[1
]}

Meyermans, H ^{[1
]}

Chen, CY ^{[1
]}

Baucher, M ^{[1
]}

Van Doorsselaere, J ^{[1
]}

Morreel, K ^{[1
]}

Messens, E ^{[1
]}

Lapierre, C ^{[1
]}

Pollet, B ^{[1
]}

Jouanin, L ^{[1
]}

Leplé, JC ^{[1
]}

Ralph, J ^{[1
]}

Marita, J ^{[1
]}

Guiney, E ^{[1
]}

Schuch, W ^{[1
]}

Petit-Conil, M ^{[1
]}

Pilate, G ^{[1
]}

机构：

[1] Univ Ghent VIB, Vakgroep Mol Genet, B-9000 Ghent, Belgium

来源：

MOLECULAR BREEDING OF WOODY PLANTS, PROCEEDINGS | 2001年 / 18卷

关键词：

CAD; CCoAOMT; COMT; Kraft pulping; lignin; poplar;

D O I：

10.1016/S0921-0423(01)80072-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transgenic poplar, downregulated in cinnamyl alcohol dehydrogenase (CAD) or caffeic acid-O-methyltransferase (COMT) expression have been grown in field trials. Wood of these trees has been evaluated for Kraft pulping. The results show that lignin is more easily extracted from wood of the CAD-downregulated trees, whereas wood from COMT-downregulated trees is less suitable for Kraft pulping. Detailed NMR analyses of lignin from COMT-downregulated poplars reveal the presence of benzodioxane structures, which are derived from coupling of 5-hydroxyconiferyl alcohol with the lignin polymer, showing that monolignols other than p-coumaryl, coniferyl and sinapyl alcohol can be incorporated into lignin. Analysis of poplar downregulated for CCoAOMT shows that CCoAOMT is involved in the synthesis of both syringyl and guaiacyl units, and that sinapic is probably not an important precursor for syringyl lignin synthesis.

引用

页码：187 / 194

页数：8

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