Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin β1 and γ1 chains

In the present study, the chain specificity of 16 commonly used monoclonal antibodies to human laminin(s) was analysed by using recombinant laminin beta 1 and gamma 1 chains. By ELISA, all antibodies reacted with purified placenta laminin, and most antibodies recognised either recombinant beta 1 or gamma 1 chains. Reactivity and chain specificity was confirmed against the recombinant chains in Western blotting under non-reducing conditions, and only a few antibodies were reactive under reducing conditions. Most antibodies were able to immunoprecipitate associated laminin beta 1/gamma 1 chains from platelet lysates. Based on these results and data from the literature, a tentative epitope map is presented. (C) 2000 Elsevier Science B.V. /International Society of Matrix Biology. AU rights reserved.