Characteristics of phospholipase D in reverse micelles of Triton X-100 and phosphatidylcholine in diethyl ether

被引：4

作者：

Subramani, S ^{[1
]}

Dittrich, N ^{[1
]}

Hirche, F ^{[1
]}

UlbrichHofmann, R ^{[1
]}

机构：

[1] UNIV HALLE WITTENBERG,DEPT BIOCHEM BIOTECHNOL,D-06099 HALLE,GERMANY

来源：

BIOTECHNOLOGY LETTERS | 1996年 / 18卷 / 07期

关键词：

D O I：

10.1007/BF00127895

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Phospholipase D, from cabbage, is active in reverse micelles formed from its substrate phosphatidylcholine and Triton X-100 in diethyl ether. The activity is optimum at w(0)=12.5. The increase of the molar ratio of Triton X-100/substrate from 1:4 to 2:1 results in an activity decrease by 25%. At 136 mM Triton X-100 the K-M value in reverse micelles is 136 mM, whereas it is 0.40 mM in the aqueous system containing SDS.

引用

页码：815 / 820

页数：6

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