ADAMs 10 and 17 Represent Differentially Regulated Components of a General Shedding Machinery for Membrane Proteins Such as Transforming Growth Factor α, L-Selectin, and Tumor Necrosis Factor α

被引:214
作者
Le Gall, Sylvain M. [1 ]
Bobe, Pierre [2 ,3 ]
Reiss, Karina [4 ]
Horiuchi, Keisuke [5 ]
Niu, Xiao-Da [6 ]
Lundell, Daniel [6 ]
Gibb, David R. [7 ]
Conrad, Daniel [7 ]
Saftig, Paul [4 ]
Blobel, Carl P. [1 ,8 ,9 ]
机构
[1] Hosp Special Surg, Arthrit & Tissue Degenerat Program, New York, NY 10021 USA
[2] CNRS, Lab Oncol Virale, F-94801 Villejuif, France
[3] Univ Paris 11, F-91405 Orsay, France
[4] Univ Kiel, Inst Biochem, D-24098 Kiel, Germany
[5] Keio Univ, Sch Med, Dept Antiaging Orthoped Res & Orthoped Surg, Tokyo 1608582, Japan
[6] Schering Plough Res Inst, Dept Inflammat, Kenilworth, NJ 07033 USA
[7] Virginia Commonwealth Univ, Dept Microbiol & Immunol, Richmond, VA 23298 USA
[8] Cornell Univ, Weill Med Coll, Dept Med, New York, NY 10021 USA
[9] Cornell Univ, Weill Med Coll, Dept Physiol & Biophys, New York, NY 10021 USA
基金
美国国家卫生研究院;
关键词
TNF-ALPHA; ECTODOMAIN; RECEPTOR; CLEAVAGE; LIGAND; EGFR; NEUTROPHILS; RELEASE; DOMAIN; ROLES;
D O I
10.1091/mbc.E08-11-1135
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Protein ectodomain shedding is a critical regulator of many membrane proteins, including epidermal growth factor receptor-ligands and tumor necrosis factor (TNF)-alpha, providing a strong incentive to define the responsible sheddases. Previous studies identified ADAM17 as principal sheddase for transforming growth factor (TGF)-alpha and heparin-binding epidermal growth factor, but Ca++ influx activated an additional sheddase for these epidermal growth factor receptor ligands in Adam17-/- cells. Here, we show that Ca++ influx and stimulation of the P2X7R signaling pathway activate ADAM10 as sheddase of many ADAM17 substrates in Adam17-/- fibroblasts and primary B cells. Importantly, although ADAM10 can shed all substrates of ADAM17 tested here in Adam17-/- cells, acute treatment of wild-type cells with a highly selective ADAM17 inhibitor (SP26) showed that ADAM17 is nevertheless the principal sheddase when both ADAMs 10 and 17 are present. However, chronic treatment of wild-type cells with SP26 promoted processing of ADAM17 substrates by ADAM10, thus generating conditions such as in Adam17-/- cells. These results have general implications for understanding the substrate selectivity of two major cellular sheddases, ADAMs 10 and 17.
引用
收藏
页码:1785 / 1794
页数:10
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