Actin-microtubule interactions in the alga Nitella: Analysis of the mechanism by which microtubule depolymerization potentiates cytochalasin's effects on streaming

被引:31
作者
Collings, DA
Wasteneys, GO
Williamson, RE
机构
关键词
actin; cytoplasmic streaming; cytochalasin; microtubule depolymerization; Nitella; oryzalin;
D O I
10.1007/BF01281816
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In the characean alga Nitella, depolymerization of microtubules potentiates the inhibitory effects of cytochalasins on cytoplasmic streaming. Microtubule depolymerization lowers the cytochalasin B and D concentrations required to inhibit streaming, accelerates inhibition and delays streaming recovery. Because microtubule depolymerization does nor significantly alter H-3-cytochalasin B uptake and release, elevated intracellular cytochalasin concentrations are not the basis for potentiation. Instead, microtubule depolymerization causes actin to become more sensitive to cytochalasin. This increased sensitivity of actin is unlikely to be due to direct stabilization of actin by microtubules, however, because very few microtubules colocalize with the subcortical actin bundles that generate streaming. Furthermore, microtubule reassembly, but nor recovery of former transverse alignment, is sufficient for restoring the normal cellular responses to cytochalasin D. We hypothesize that either tubulin or microtubule-associated proteins, released when microtubules depolymerize, interact with the actin cytoskeleton and sensitize it to cytochalasin.
引用
收藏
页码:178 / 190
页数:13
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