Immunolocalization of potassium-chloride cotransporter polypeptides in rat exocrine glands

被引：21

作者：

Roussa, E

Shmukler, BE

Wilhelm, S

Casula, S

Stuart-Tilley, AK

Thévenod, F

Alper, SL ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Med, Boston, MA 02215 USA

[2] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02215 USA

[3] Univ Gottingen, Dept Neuroanat, D-3400 Gottingen, Germany

[4] Beth Israel Deaconess Med Ctr, Mol Med Unit, Boston, MA 02215 USA

[5] Beth Israel Deaconess Med Ctr, Renal Unit, Boston, MA 02215 USA

[6] Univ Manchester, Sch Biol Sci, Manchester M13 9PL, Lancs, England

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2002年 / 117卷 / 04期

关键词：

potassium-chloride cotransport; salivary secretion; pancreatic secretion; exocrine glands; immunofluorescence microscopy; immunoblot;

D O I：

10.1007/s00418-002-0393-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Potassium-chloride cotransporters (KCCs) encoded by at least four homologous genes are believed to contribute to cell volume regulation and transepithelial ion transport. We have studied KCC polypeptide expression and immunolocalization of KCCs in rat salivary glands and pancreas. Immunoblot analysis of submandibular, parotid, and pancreas plasma membrane fractions with immunospecific antibodies raised against mouse KCC1 revealed protein bands at ca 135 kDa and ca 150 kDa. Immunocytochemical analysis of fixed salivary and pancreas tissue revealed basolateral KCC1 distribution in rat parotid and pancreatic acinar cells, as well as in parotid, submandibular, and pancreatic duct cells. KCC1 or the polypeptide product(s) of one or more additional KCC genes was also expressed in the basolateral membranes of submandibular acinar cells. Both immunoblot and immunofluorescence signals were abolished in the presence of the peptide antigen. These results establish the presence in rat exocrine glands of KCC1 and likely other KCC polypeptides, and suggest a contribution of KCC polypeptides to transepithelial Cl- transport.

引用

页码：335 / 344

页数：10

共 39 条

[1] CL--DEPENDENT NH4+ TRANSPORT MECHANISMS IN MEDULLARY THICK ASCENDING LIMB CELLS [J].