Low-cost and high-throughput Multi-Channel Capillary Electrophoresis (MCCE) system for DNA analysis

被引：8

作者：

Amirkhanian, VD ^{[1
]}

Liu, MS ^{[1
]}

机构：

[1] BioCal Technol Inc, Orange, CA 92867 USA

来源：

BIOMEDICAL NANOTECHNOLOGY ARCHITECTURES AND APPLICATIONS | 2002年 / 4626卷

关键词：

capillary electrophoresis; fluorescence detection; optical fibers; genomics; DNA fragment analysis;

D O I：

10.1117/12.472087

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this paper we present the innovative use of an inexpensive Multi-Channel Capillary-based Electrophoresis (MCCE) system in combination with disposable separation cartridge for routine analysis of DNA fragments. The proposed multi-channel system is based on a novel multiplexed fluorescence detection technology, which provides a rapid and unique solution for DNA analysis. Presently the Capillary Electrophoresis (CE) based technology used for DNA analysis, rely on gas discharge UV-Visible lamps or lasers as light sources that are bulky, expensive, and difficult to couple one's light output into optical fibers, for miniaturization of the optical detection system. The light sources hinder the development of small sized, high-throughput and cost-effective genomics instrument. Whereas, the proposed instrument with solid-state light sources and non-moving detection micro-optics, and re-usable cartridge containing multiple separation channels, provide a cost-effective solution for a robust CE instrument. Furthermore, the simplified operation of the MCCE instrument will drastically reduce the cost of DNA analysis, and possibly will be the instrument of choice for forensic DNA and molecular diagnostics applications in the near future.

引用

页码：238 / 246

页数：9

共 21 条

[1]

Bashkin JS, 1996, J CAPILLARY ELECTROP, V3, P61

[2] HIGH-SPEED PARALLEL SEPARATION OF DNA RESTRICTION FRAGMENTS USING CAPILLARY ARRAY ELECTROPHORESIS [J].