Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection

被引：46

作者：

Zhang, ZY

Xu, JS

Xu, QJ

Larkin, P

Xin, ZY ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Crop Breeding & Cultivat, Minist Agr, Key Lab Crop Genet & Breeding, Beijing 100081, Peoples R China

[2] CSIRO Plant Ind, Canberra, ACT 2601, Australia

来源：

THEORETICAL AND APPLIED GENETICS | 2004年 / 109卷 / 02期

关键词：

D O I：

10.1007/s00122-004-1649-1

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The distal segment of the long arm of the Thinopyrum intermedium chromosome 7Ai1 carries the barley yellow dwarf virus (BYDV) resistance gene Bdv2. This segment was transferred to the distal region of the long arm of wheat chromosome 7D in the Yw series of translocation lines by using the ph1b mutant to induce homoeologous pairing. To transfer Bdv2 to commercial varieties, we developed two resistance gene-analog polymorphism (RGAP) markers, Tgp-1(350) and Tgp-2(210), and one randomly amplified polymorphic DNA (RAPD) marker, OPD04(1300). The diagnostic fragments of the RGAP marker Tgp-1(350) and the RAPD marker OPD04(1300) were cloned, sequenced and converted into sequence-characterized amplified region (SCAR) markers, named SC-gp1 and SC-D04, respectively. SC-gp1 and SC-D04 were validated based on available translocation lines and segregating F-2 individuals. Our results indicated that the SCAR markers co-segregated with the BYDV resistance associated with Bdv2. Therefore, they can be used as a low-cost, high-throughput alternative to conventional phenotypic screening in wheat-breeding programs exploiting Bdv2. The marker-assisted selection for BYDV resistance was successfully performed in a wheat-breeding program.

引用

页码：433 / 439

页数：7

共 34 条

[1] A diagnostic molecular marker allowing the study of Th. intermedium-derived resistance to BYDV in bread wheat segregating populations [J].

Ayala, L ;

Henry, M ;

González-de-León, D ;

van Ginkel, M ;

Mujeeb-Kazi, A ;

Keller, B ;

Khairallah, M .

THEORETICAL AND APPLIED GENETICS, 2001, 102 (6-7) :942-949

[2] THE USE OF CELL-CULTURE FOR SUBCHROMOSOMAL INTROGRESSIONS OF BARLEY YELLOW DWARF VIRUS-RESISTANCE FROM THINOPYRUM-INTERMEDIUM TO WHEAT [J].

BANKS, PM ;

LARKIN, PJ ;

BARIANA, HS ;

LAGUDAH, ES ;

APPELS, R ;

WATERHOUSE, PM ;

BRETTELL, RIS ;

CHEN, X ;

XU, HJ ;

XIN, ZY ;

QIAN, YT ;

ZHOU, XM ;

CHENG, ZM ;

ZHOU, GH .

GENOME, 1995, 38 (02) :395-405

[3]

BANKS PM, 1996, 5 INT WHEAT C 10 14, P114

[4] A SINGLE WHEATGRASS CHROMOSOME REDUCES THE CONCENTRATION OF BARLEY YELLOW DWARF VIRUS IN WHEAT [J].

BRETTELL, RIS ;

BANKS, PM ;

CAUDERON, Y ;

CHEN, X ;

CHENG, ZM ;

LARKIN, PJ ;

WATERHOUSE, PM .

ANNALS OF APPLIED BIOLOGY, 1988, 113 (03) :599-603

[5]

Cauderon Y., 1973, Proceedings of the fourth international wheat genetics symposium. Pests., P401

[6] Recessive genes for resistance to Puccinia striiformis f. sp. hordei in barley [J].

Chen, XM ;

Line, RF .

PHYTOPATHOLOGY, 1999, 89 (03) :226-232

[7] Genome scanning for resistance-gene analogs in rice, barley, and wheat by high-resolution electrophoresis [J].

Chen, XM ;

Line, RF ;

Leung, H .

THEORETICAL AND APPLIED GENETICS, 1998, 97 (03) :345-355

[8]

Conti M. C., 1990, World Perspectives on Barley yellow dwarf, P1

[9] Molecular cloning of a new receptor-like kinase gene encoded at the Lr10 disease resistance locus of wheat [J].

Feuillet, C ;

Schachermayr, G ;

Keller, B .

PLANT JOURNAL, 1997, 11 (01) :45-52

[10] C-BANDING AND INSITU HYBRIDIZATION ANALYSES OF AGROPYRON-INTERMEDIUM, A PARTIAL WHEAT X AG INTERMEDIUM AMPHIPLOID, AND 6 DERIVED CHROMOSOME ADDITION LINES [J].

FRIEBE, B ;

MUKAI, Y ;

GILL, BS ;

CAUDERON, Y .

THEORETICAL AND APPLIED GENETICS, 1992, 84 (7-8) :899-905

← 1 2 3 4 →