Internal validation of screening methodology for residues of epi-trenbolone, trenbolone, epi-nortestosterone and nortestosterone in bovine urine using immunoaffinity cartridges for sample clean-up

被引：7

作者：

Dickson, LC ^{[1
]}

MacNeil, JD ^{[1
]}

Reid, J ^{[1
]}

Neiser, CD ^{[1
]}

Fesser, ACE ^{[1
]}

机构：

[1] Ctr Vet Drug Residues, Canadian Food Inspect Agcy, Saskatoon Lab, Saskatoon, SK S7N 2R3, Canada

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 473卷 / 1-2期

关键词：

bovine urine; hormones; immunoaffinity; screening; validation;

D O I：

10.1016/S0003-2670(02)00974-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This multi-analyte procedure uses commercially available immunoaffinity chromatography (IAC) clean-up cartridges for extraction and clean-up, followed by determination of residues of epi-trenbolone (ETB), trenbolone (TB), epi-nortestosterone (epi-nandrolone) (ENT) and nortestosterone (nandrolone) (NT) in bovine urine by liquid-chromatography with UV absorbance detection. This single-laboratory internal validation included assessment of recoveries, repeatability, linearity, detection capability and cross-reactivity (specificity) with a suite of antibiotics and other hormonal growth promotants (HGPs). The method was validated for screening at a target level of 2.0 ng ml(-1) (C) 2002 Published by Elsevier Science B.V.

引用

页码：111 / 118

页数：8

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