The hepatitis B virus X-associated protein, XAP3, is a protein kinase C-binding protein

被引:57
作者
Cong, YS [1 ]
Yao, YL [1 ]
Yang, WM [1 ]
Kuzhandaivelu, N [1 ]
Seto, E [1 ]
机构
[1] UNIV S FLORIDA, COLL MED, DEPT MED MICROBIOL & IMMUNOL, MOL ONCOL PROGRAM, TAMPA, FL 33612 USA
关键词
D O I
10.1074/jbc.272.26.16482
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The hepatitis B virus X protein induces transcriptional activation of a wide variety of viral and cellular genes. In addition to its ability to interact directly with many nuclear transcription factors, several reports indicate that the X protein stimulates different cytoplasmic kinase signal cascades. Using the yeast two-hybrid screen, we have isolated a clone designated X-associated protein 3 (XAP3) that encodes a human homolog of the rat protein kinase C-binding protein. One of the activation domains of X (amino acids 90-122) is required for binding to XAP3, while the NH2-terminal part of XAP3 is necessary for binding to X. Both X and XAP3 bound specifically to the eta PKC isoenzyme synthesized in rabbit reticulocyte lysates. Overexpression of XAP3 enhanced X transactivation activity. These results support earlier findings that one of the mechanisms of transactivation by X is through involvement with the cellular protein kinase C pathway.
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收藏
页码:16482 / 16489
页数:8
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