Purification of virus-like particles of recombinant human papillomavirus type 11 major capsid protein L1 from Saccharomyces cerevisiae

被引:95
作者
Cook, JC
Joyce, JG
George, HA
Schultz, LD
Hurni, WM
Jansen, KU
Hepler, RW
Ip, C
Lowe, RS
Keller, PM
Lehman, ED
机构
[1] Department of Virus and Cell Biology, Merck Research Laboratories, West Point
[2] Dept. Vacc. BioProcess Research D., Merck Research Laboratories, West Point
关键词
D O I
10.1006/prep.1999.1155
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Recombinant major capsid protein, L1 (M-r = 55,000), of human papillomavirus type 11 was expressed intracellularly at high levels in a galactose-inducible Saccharomyces cerevisiae expression system by an HPV6/11 hybrid gene. The capsid protein self-assembled into virus-like particles (VLPs) and accounted for 15% of the total soluble protein. A purification process was developed that consisted of two main steps: microfiltration and cation-exchange chromatography. The purified VLPs were 98% homogeneous, and the overall purification yield was 10%. The final product was characterized by several analytical methods and was highly immunogenic in mice. (C) 1999 Academic Press.
引用
收藏
页码:477 / 484
页数:8
相关论文
共 30 条
  • [1] STRUCTURES OF BOVINE AND HUMAN PAPILLOMAVIRUSES - ANALYSIS BY CRYOELECTRON MICROSCOPY AND 3-DIMENSIONAL IMAGE-RECONSTRUCTION
    BAKER, TS
    NEWCOMB, WW
    OLSON, NH
    COWSERT, LM
    OLSON, C
    BROWN, JC
    [J]. BIOPHYSICAL JOURNAL, 1991, 60 (06) : 1445 - 1456
  • [2] EXPRESSION, PURIFICATION AND CHARACTERIZATION OF RECOMBINANT HUMAN INSULIN-LIKE GROWTH FACTOR-I IN YEAST
    BAYNE, ML
    APPLEBAUM, J
    CHICCHI, GG
    HAYES, NS
    GREEN, BG
    CASCIERI, MA
    [J]. GENE, 1988, 66 (02) : 235 - 244
  • [3] THE FOCUSING POSITIONS OF POLYPEPTIDES IN IMMOBILIZED PH GRADIENTS CAN BE PREDICTED FROM THEIR AMINO-ACID-SEQUENCES
    BJELLQVIST, B
    HUGHES, GJ
    PASQUALI, C
    PAQUET, N
    RAVIER, F
    SANCHEZ, JC
    FRUTIGER, S
    HOCHSTRASSER, D
    [J]. ELECTROPHORESIS, 1993, 14 (10) : 1023 - 1031
  • [4] REFERENCE POINTS FOR COMPARISONS OF 2-DIMENSIONAL MAPS OF PROTEINS FROM DIFFERENT HUMAN CELL-TYPES DEFINED IN A PH SCALE WHERE ISOELECTRIC POINTS CORRELATE WITH POLYPEPTIDE COMPOSITIONS
    BJELLQVIST, B
    BASSE, B
    OLSEN, E
    CELIS, JE
    [J]. ELECTROPHORESIS, 1994, 15 (3-4) : 529 - 539
  • [5] PREVALENCE OF HUMAN PAPILLOMAVIRUS IN CERVICAL-CANCER - A WORLDWIDE PERSPECTIVE
    BOSCH, FX
    MANOS, MM
    MUNOZ, N
    SHERMAN, M
    JANSEN, AM
    PETO, J
    SCHIFFMAN, MH
    MORENO, V
    KURMAN, R
    SHAH, KV
    ALIHONOU, E
    BAYO, S
    MOKHTAR, HC
    CHICAREON, S
    DAUDT, A
    DELOSRIOS, E
    GHADIRIAN, P
    KITINYA, JN
    KOULIBALY, M
    NGELANGEL, C
    TINTORE, LMP
    RIOSDALENZ, JL
    SARJADI
    SCHNEIDER, A
    TAFUR, L
    TEYSSIE, AR
    ROLON, PA
    TORROELLA, M
    TAPIA, AV
    WABINGA, HR
    ZATONSKI, W
    SYLLA, B
    VIZCAINO, P
    MAGNIN, D
    KALDOR, J
    GREER, C
    WHEELER, C
    [J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1995, 87 (11): : 796 - 802
  • [6] ANALYSIS OF HUMAN PAPILLOMAVIRUS TYPES IN EXOPHYTIC CONDYLOMATA ACUMINATA BY HYBRID CAPTURE AND SOUTHERN BLOT TECHNIQUES
    BROWN, DR
    BRYAN, JT
    CRAMER, H
    FIFE, KH
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (10) : 2667 - 2673
  • [7] ASSEMBLED BACULOVIRUS-EXPRESSED HUMAN PAPILLOMAVIRUS TYPE-11 L1 CAPSID PROTEIN VIRUS-LIKE PARTICLES ARE RECOGNIZED BY NEUTRALIZING MONOCLONAL-ANTIBODIES AND INDUCE HIGH TITERS OF NEUTRALIZING ANTIBODIES
    CHRISTENSEN, ND
    HOPFL, R
    DIANGELO, SL
    CLADEL, NM
    PATRICK, SD
    WELSH, PA
    BUDGEON, LR
    REED, CA
    KREIDER, JW
    [J]. JOURNAL OF GENERAL VIROLOGY, 1994, 75 : 2271 - 2276
  • [8] de Villiers E M, 1994, Curr Top Microbiol Immunol, V186, P1
  • [9] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES
    DUBOIS, M
    GILLES, KA
    HAMILTON, JK
    REBERS, PA
    SMITH, F
    [J]. ANALYTICAL CHEMISTRY, 1956, 28 (03) : 350 - 356
  • [10] FRINGS CS, 1970, AM J CLIN PATHOL, V53, P89