K depletion and NH4Cl acidosis increase apical insertion of studded membrane in type A intercalated cells

被引:9
作者
Narbaitz, R
Murugesan, S
Levine, DZ
机构
[1] UNIV OTTAWA, DEPT ANAT & NEUROBIOL, OTTAWA, ON K1H 8M5, CANADA
[2] UNIV OTTAWA, DIV NEPHROL, OTTAWA, ON K1H 8M5, CANADA
关键词
acid base; intercalated cells; acidosis; hydrogen ion adenosinetriphosphatase; bicarbonate;
D O I
10.1152/ajprenal.1996.270.4.F649
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
To examine the effects of K depletion and metabolic acidosis on the prevalence and distribution of H+-adenosinetriphosphatase (H+-ATPase)-related studs at the luminal plasma membrane of A-type intercalated cells (A-ICs), we conducted a quantitative electron microscopical study on the cortical collecting ducts (CCDs) of control, NH4Cl-loaded, and K-depleted rats. The percentage of A-ICs was slightly increased in the K-depleted but not in the acidotic rats. A-ICs were considered ''active'' when they presented a semicontinuous row of 9- to 10-nm studs at the cytoplasmic face of the apical membrane and ''inactive'' when all of the apical membrane was devoid of studs. The percentage of active A-ICs was greatly increased in acidotic (87.2%) and K-depleted (79.3%) rats compared with controls (41.6%). These results give a quantitative expression to the general view that acidosis elicits insertion of studded membrane in the apical plasma membrane of A-ICs. Furthermore, we show, for the first time, that an increase in the membrane insertion of H+-ATPase is also part of the response to K depletion.
引用
收藏
页码:F649 / F656
页数:8
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