Effects of chilling on male gametophyte development in rice

被引：136

作者：

Mamun, E. A.

Alfred, S.

Cantrill, L. C.

Overall, R. L.

Sutton, B. G.

机构：

[1] Univ Sydney, Fac Agr Food & Nat Resources, Sydney, NSW 2006, Australia

[2] Univ Sydney, Cooperat Res Ctr Sustainable Rice Prod, Sydney, NSW 2006, Australia

[3] Univ Sydney, Sch Biol Sci, Sydney, NSW 2006, Australia

来源：

CELL BIOLOGY INTERNATIONAL | 2006年 / 30卷 / 07期

关键词：

anther; callose; carbohydrate metabolism; chilling; microspores wall; rice; RNA in situ hybridisation; OsMST8; starch accumulation; ultrastructure;

D O I：

10.1016/j.cellbi.2006.03.004

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Chilling during male gametophyte development in rice inhibits development of microspores, causing male sterility. Changes in cellular ultrastructure that have been exposed to mild chilling include microspores with poor pollen wall formation, abnormal vacuolation and hypertrophy of the tapetum and unusual starch accumulation in the plastids of the endothecium in post-meiotic anthers. Anthers observed during tetrad release also have callose (1,3-beta-glucan) wall abnormalities as shown by immunocytochemical labelling. Expression of rice anther specific monosaccharide transporter (OsMST8) is greatly affected by chilling treatment. Perturbed carbohydrate metabolism, which is particularly triggered by repressed genes OsINV4 and OsMST8 during chilling, causes unusual starch storage in the endothecium and this also contributes to other symptoms such as vacuolation and poor microspore wall formation. Premature callose breakdown apparently restricts the basic framework of the future pollen wall. Vacuolation and hypertrophy are also symptoms of osmotic imbalance triggered by the reabsorption of callose breakdown products due to absence of OsMST8 activity. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：583 / 591

页数：9

共 42 条

[1] Cellular organisation in meiotic and early post-meiotic rice anthers [J].