A chalcone synthase/stilbene synthase DNA probe for conifers

被引：7

作者：

Baker, SM ^{[1
]}

White, EE ^{[1
]}

机构：

[1] FORESTRY CANADA,PACIFIC FORESTRY CTR,NAT RESOURCES CANADA,VICTORIA,BC V8Z 1M5,CANADA

来源：

THEORETICAL AND APPLIED GENETICS | 1996年 / 92卷 / 07期

关键词：

Pinus monticola D Don; chalcone synthase; stillbene synthase; DNA probes; conifers;

D O I：

10.1007/BF00221894

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A probe for chalcone synthase (CHS) was generated by PCR using chalcone synthase conserved sequences. The cloned PCR product has high similarity to both chalcone synthase and stilbene synthase sequences. The probe was used to examine the organization of chalcone synthase and stilbene synthase genes in Abies procera, Pinus lambertiana, P. monticola, Picea glauca, P. sitchensis, Pseudostuga menziesii, Taxus brevifolia, and Thuja plicata. A large number of hybridizing bands were found in all species except T. plicata which did not cross hybridize. The hybridization patterns are highly polymorphic between the species and are also polymorphic within several of them.

引用

页码：827 / 831

页数：5

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