Microbial elicitors induce activation and dual phosphorylation of the Arabidopsis thaliana MAPK 6

被引:243
作者
Nühse, TS
Peck, SC
Hirt, H
Boller, T
机构
[1] Friedrich Miescher Inst, CH-4058 Basel, Switzerland
[2] Univ Vienna, Vienna Bioctr, Inst Genet & Microbiol, A-1030 Vienna, Austria
关键词
D O I
10.1074/jbc.275.11.7521
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protein kinases related to the family of mitogen-activated kinases (MAPKs) have been established as signal transduction components in a variety of processes in plants. For Arabidopsis thaliana, however, although one of the genetically best studied plant species, biochemical data on activation of mitogen-activated protein kinases are lacking. A. thaliana MAPK 6 (AtMPK6) is the Arabidopsis orthologue of a tobacco MAPK termed salicylate-induced protein kinase, which is activated by general and race-specific elicitors as well as by physical stress. Using a C terminus-specific antibody, we show that AtMPK6 is activated in elicitor-treated cell cultures of A. thaliana. Four different elicitors from bacteria, fungi, and plants lead to a rapid and transient activation of AtMPK6, indicating a conserved signaling pathway. The induction was equally rapid as medium alkalinization, one of the earliest elicitor response observed in cell cultures. A similarly rapid activation of AtMPK6 was observed in elicitor-treated leaf strips, demonstrating that recognition of the elicitors and activation of the MAPK pathway occurs also in intact plants. We demonstrate by in vivo labeling that AtMPK6 is phosphorylated on threonine and tyrosine residues in elicited cells.
引用
收藏
页码:7521 / 7526
页数:6
相关论文
共 53 条
  • [1] Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis
    Aarts, N
    Metz, M
    Holub, E
    Staskawicz, BJ
    Daniels, MJ
    Parker, JE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (17) : 10306 - 10311
  • [2] Rapid and transient activation of a myelin basic protein kinase in tobacco leaves treated with harpin from Erwinia amylovora
    Adam, AL
    Pike, S
    Hoyos, ME
    Stone, JM
    Walker, JC
    Novacky, A
    [J]. PLANT PHYSIOLOGY, 1997, 115 (02) : 853 - 861
  • [3] INACTIVATION OF P42 MAP KINASE BY PROTEIN PHOSPHATASE 2A AND A PROTEIN-TYROSINE-PHOSPHATASE, BUT NOT CL100, IN VARIOUS CELL-LINES
    ALESSI, DR
    GOMEZ, N
    MOORHEAD, C
    LEWIS, T
    KEYSE, SM
    COHEN, P
    [J]. CURRENT BIOLOGY, 1995, 5 (03) : 283 - 295
  • [4] BISHOP PD, 1984, J BIOL CHEM, V259, P3172
  • [5] A MAP kinase is activated late in plant mitosis and becomes localized to the plane of cell division
    Bögre, L
    Calderini, O
    Binarova, P
    Mattauch, M
    Till, S
    Kiegerl, S
    Jonak, C
    Pollaschek, C
    Barker, P
    Huskisson, NS
    Hirt, H
    Heberle-Bors, E
    [J]. PLANT CELL, 1999, 11 (01) : 101 - 113
  • [6] Bogre L, 1997, PLANT CELL, V9, P75, DOI 10.1105/tpc.9.1.75
  • [7] Mechanosensors in plants
    Bogre, L
    Ligterink, W
    HeberleBors, E
    Hirt, H
    [J]. NATURE, 1996, 383 (6600) : 489 - 490
  • [8] BOLLER T, 1995, ANNU REV PLANT PHYS, V46, P189, DOI 10.1146/annurev.arplant.46.1.189
  • [9] BOYLE WJ, 1991, METHOD ENZYMOL, V201, P110
  • [10] Calderini O, 1998, J CELL SCI, V111, P3091