Biochemical and functional characterization of the cis-spliceosomal U1 small nuclear RNP from Trypanosoma brucei

被引：20

作者：

Palfi, Z

Lane, WS

Bindereif, A

机构：

[1] Univ Giessen, Inst Biochem, D-35392 Giessen, Germany

[2] Harvard Univ, Harvard Microchem & Proteom Anal Facil, Cambridge, MA 02138 USA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2002年 / 121卷 / 02期

关键词：

small nuclear RNA; snRNA; snRNP; splicing; trypanosomes;

D O I：

10.1016/S0166-6851(02)00044-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies in the trypanosome system have revealed that in addition to trans splicing of a short spliced leader (SL) exon, there is also cis splicing of internal introns. It has been suggested that cis splicing requires base-pairing of U1 small nuclear RNA (snRNA) and the 5' splice site. We have cloned the gene for U I snRNA from Trypanosoma brucei and characterized the U I snRNP. Based on immunoprecipitation and direct mass-spectrometric protein analysis the U1 snRNP contains the common Sm core found also in the known trans-spliceosomal snRNPs U2, U4/U6, and U5. The 5' end of U I snRNA in the U I snRNP is accessible for and functional in specific recognition of the 5' splice site of the poly(A) polymerase intron. (C) 2002 Elsevier Science B.V. All rights reserved.

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页码：233 / 243

页数：11

相关论文

共 42 条

[1] Natural trans-splicing in carnitine octanoyltransferase pre-mRNAs in rat liver [J].

Caudevilla, C ;

Serra, D ;

Miliar, A ;

Codony, C ;

Asins, G ;

Bach, M ;

Hegardt, FG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12185-12190

[2] Rabbit β-globin is extended beyond its UGA stop codon by multiple suppressions and translational reading gaps [J].

Chittum, HS ;

Lane, WS ;

Carlson, BA ;

Roller, PP ;

Lung, FD ;

Lee, BJ ;

Hatfield, DL .

BIOCHEMISTRY, 1998, 37 (31) :10866-10870

[3] ANALYSIS OF SMALL NUCLEAR RIBONUCLEOPROTEINS (RNPS) IN TRYPANOSOMA-BRUCEI - STRUCTURAL ORGANIZATION AND PROTEIN-COMPONENTS OF THE SPLICED LEADER RNP [J].

CROSS, M ;

GUNZL, A ;

PALFI, Z ;

BINDEREIF, A .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (11) :5516-5526

[4] THE TRANS-SPLICEOSOMAL U2 SNRNP PROTEIN 40K OF TRYPANOSOMA-BRUCEI - CLONING AND ANALYSIS OF FUNCTIONAL DOMAINS REVEALS HOMOLOGY TO A MAMMALIAN SNRNP PROTEIN [J].

CROSS, M ;

WIELAND, B ;

PALFI, Z ;

GUNZL, A ;

ROTHLISBERGER, U ;

LAHM, HW ;

BINDEREIF, A .

EMBO JOURNAL, 1993, 12 (03) :1239-1248

[5] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[6] Characterization of a candidate Trypanosoma brucei U1 small nuclear RNA gene [J].

Djikeng, A ;

Ferreira, L ;

D'Angelo, M ;

Dolezal, P ;

Lamb, T ;

Murta, S ;

Triggs, V ;

Ulbert, S ;

Villarino, A ;

Renzi, S ;

Ullu, E ;

Tschudi, C .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2001, 113 (01) :109-115

[7] Evidence for the presence of a small U5-like RNA in active trans-spliceosomes of Trypanosoma brucei [J].

Dungan, JM ;

Watkins, KP ;

Agabian, N .

EMBO JOURNAL, 1996, 15 (15) :4016-4029

[8] AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE [J].

ENG, JK ;

MCCORMACK, AL ;

YATES, JR .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) :976-989

[9] Exon repetition in mRNA [J].

Frantz, SA ;

Thiara, AS ;

Lodwick, D ;

Ng, LL ;

Eperon, IC ;

Samani, NJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (10) :5400-5405

[10] Purification of the spliced leader ribonucleoprotein particle from Leptomonas collosoma revealed the existence of an Sm protein in trypanosomes -: Cloning the SmE homologue [J].

Goncharov, I ;

Palfi, Z ;

Bindereif, A ;

Michaeli, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (18) :12217-12221

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