Biased T cell receptor V gene usage in tissues with periodontal disease

被引:29
作者
Nakajima, T [1 ]
Yamazaki, K [1 ]
Hara, K [1 ]
机构
[1] NIIGATA UNIV,SCH DENT,DEPT PERIODONTOL,NIIGATA 951,JAPAN
关键词
T-cell receptor; periodontitis; gingivitis; immunohistology; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; HEAT-SHOCK PROTEINS; RHEUMATOID-ARTHRITIS; MONOCLONAL-ANTIBODY; BETA-GENES; HUMAN SKIN; EXPRESSION; LYMPHOCYTES; HETEROGENEITY; RECOGNITION;
D O I
10.1111/j.1600-0765.1996.tb00457.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
In an attempt to characterize TCR V gene usage in human periodontally diseased tissue, V alpha 2, V beta 5.2-3, V beta 5.3, V beta 5.1, V beta 6.7, V beta 8 and V beta 12.1 expressions were examined. Serial cryostat sections obtained from 20 periodontitis and 9 gingivitis biopsies were then reacted with monoclonal antibodies directed to each repertoire. The technique was combined with a sensitive alkaline phosphatase-anti-alkaline phosphatase method. Peripheral blood was obtained from 10 periodontitis and 2 gingivitis patients. TCR repertoire was also quantified by flow cytofluorography with FITC-conjugated antibodies. Cells displaying binding of each antibody were counted. The proportions to CD3-positive cells were then calculated. The pattern of each TCR V gene product expression in inflamed gingiva exhibited individual variation, nevertheless, a consistent pattern emerged. The V beta 5 subfamily and V beta 6.7 were frequently used repertoires in gingiva, whereas the V alpha 2 and V beta 8 subfamily were underexpressed in most cases. Furthermore, the TCR V gene product expression in gingival tissue was biased compared with autologous peripheral blood. Three of 10 periodontitis subjects showed 1 or 2 strikingly overrepresented repertoire comparatively with autologous blood. In these 3 subjects V beta 6.7 was overexpressed in two cases and 5.2-3, V beta 8 and V beta 12.1 were overexpressed in one case. These results suggest that gingival T-cells are not randomly mobilized from peripheral blood and that local events influence the TCR repertoire at the level of T-cell recruitment or T-cell expansion.
引用
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页码:2 / 10
页数:9
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