Antiviral activity and underlying molecular mechanisms of Matrine against porcine reproductive and respiratory syndrome virus in vitro

被引：93

作者：

Sun, Na ^{[1
]}

Wang, Zhi-Wei ^{[1
]}

Wu, Cai-Hong ^{[2
]}

Li, E. ^{[1
]}

He, Jun-Ping ^{[1
]}

Wang, Shao-Yu ^{[3
]}

Hu, Yuan-Liang ^{[4
]}

Lei, Hai-Min ^{[5
]}

Li, Hong-Quan ^{[1
]}

机构：

[1] Shanxi Agr Univ, Coll Anim Sci & Vet Med, Taigu 030801, Shanxi, Peoples R China

[2] Jiangsu Anim Husb & Vet Coll, Taizhou 225300, Jiangsu, Peoples R China

[3] Univ Western Sydney, Sch Med, Penrith, NSW 2751, Australia

[4] Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Jiangsu, Peoples R China

[5] Beijing Univ Chinese Med, Sch Chinese Pharm, Beijing 100102, Peoples R China

来源：

RESEARCH IN VETERINARY SCIENCE | 2014年 / 96卷 / 02期

关键词：

Matrine; Antiviral; PRRSV; N protein; Apoptosis; MEDICINE MATRINE; APOPTOSIS; PROTEIN; PRRSV;

D O I：

10.1016/j.rvsc.2013.12.009

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

090604 [动物药学];

摘要：

Porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV), is an acute infectious disease. The prevalence of PRRS has made swine industry suffered huge financial losses. Matrine, a natural compound, has been demonstrated to possess anti PRRSV activity in Marc-145 cells. However, the underlying molecular mechanisms were still unknown. The main objective of our study was to discuss the effect of Matrine on PRRSV N protein expression and PRRSV induced apoptosis. Indirect immunofluorescence assay (IFA) and Western blot were used to assess the effect of Matrine on N protein expression. Apoptosis was analyzed by fluorescence staining. In addition, the effect of Matrine on caspase-3 activation was investigated by Western blot. Indirect immunofluorescence assay and Western blot analysis demonstrated that Matrine could inhibit N protein expression in Marc-145 cells. And Matrine was found to be able to impair PRRSV-induced apoptosis by inhibiting caspase-3 activation. (C) 2014 Published by Elsevier Ltd.

引用

页码：323 / 327

页数：5

共 23 条

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