Roughness response genes in osteoblasts

被引:182
作者
Brett, PM
Harle, J
Salih, V
Mihoc, R
Olsen, I
Jones, FH
Tonetti, M
机构
[1] UCL, Eastman Dent Inst, Dept Periodontol, London WC1X 8LD, England
[2] UCL, Eastman Dent Inst, Dept Biomat, London WC1X 8LD, England
[3] UCL, Dept Chem, Christopher Ingold Labs, London WC1H 0AJ, England
基金
英国工程与自然科学研究理事会;
关键词
roughness; titanium; bone; cells; genes;
D O I
10.1016/j.bone.2004.03.009
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Titanium (Ti) and Ti alloys are widely used as dental and orthopedic implants, but the effects of the surface characteristics of these materials, including roughness, on the response of target tissues in vivo are not well understood. The present study has therefore examined the effects of a moderately rough (sand-blasted, acid-etched; SLA) Ti surface, a highly rough (plasma-sprayed; TPS) surface, and a smooth surface (SMO) on bone cells in vitro. X-ray photoelectron spectroscopy showed that these surfaces had similar surface chemistry, while scanning electron microscopy suggested that the SLA provided a transiently less biocompatible surface, with initially less well-attached cells. SLA also delayed bone cell growth compared with SMO, whereas the TPS surface elicited the greatest increase in cell numbers. In addition, expression profiling using the ATLAS(TM) gene array showed marked differences in gene responses after 3 h of incubation, which increased further after 24 It, with TPS generating the largest number of up- and down-regulated genes compared with SLA and SMO. A number of osteoblast genes were also identified as 'roughness' genes on the basis of their similar response on SLA and TPS, compared with SMO. These findings thus show, for the first time, that the surface roughness of Ti has a profound effect on the profile of genes expressed by bone cells and suggest that improvements in the biological activity and possibly the clinical efficacy of these materials could be achieved by selective regulation of gene expression mediated via modification of surface roughness. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:124 / 133
页数:10
相关论文
共 32 条
[1]   A 15-YEAR STUDY OF OSSEOINTEGRATED IMPLANTS IN THE TREATMENT OF THE EDENTULOUS JAW [J].
ADELL, R ;
LEKHOLM, U ;
ROCKLER, B ;
BRANEMARK, PI .
INTERNATIONAL JOURNAL OF ORAL SURGERY, 1981, 10 (06) :387-416
[2]   THE SURFACE-ANALYSIS OF IMPLANT MATERIALS .1. THE SURFACE-COMPOSITION OF A TITANIUM DENTAL IMPLANT MATERIAL [J].
AMEEN, AP ;
SHORT, RD ;
JOHNS, R ;
SCHWACH, G .
CLINICAL ORAL IMPLANTS RESEARCH, 1993, 4 (03) :144-150
[3]   Effect of grooved titanium substratum on human osteoblastic cell growth [J].
Anselme, K ;
Bigerelle, M ;
Noël, B ;
Iost, A ;
Hardouin, P .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 2002, 60 (04) :529-540
[4]   Osteoblast-mediated mineral deposition in culture is dependent on surface microtopography [J].
Boyan, BD ;
Bonewald, LF ;
Paschalis, EP ;
Lohmann, CH ;
Rosser, J ;
Cochran, DL ;
Dean, DD ;
Schwartz, Z ;
Boskey, AL .
CALCIFIED TISSUE INTERNATIONAL, 2002, 71 (06) :519-529
[5]   Surface roughness mediates its effects on osteoblasts via protein kinase A and phospholipase A2 [J].
Boyan, BD ;
Sylvia, VL ;
Liu, YH ;
Sagun, R ;
Cochran, DL ;
Lohmann, CH ;
Dean, DD ;
Schwartz, Z .
BIOMATERIALS, 1999, 20 (23-24) :2305-2310
[6]   Minimum information about a microarray experiment (MIAME) - toward standards for microarray data [J].
Brazma, A ;
Hingamp, P ;
Quackenbush, J ;
Sherlock, G ;
Spellman, P ;
Stoeckert, C ;
Aach, J ;
Ansorge, W ;
Ball, CA ;
Causton, HC ;
Gaasterland, T ;
Glenisson, P ;
Holstege, FCP ;
Kim, IF ;
Markowitz, V ;
Matese, JC ;
Parkinson, H ;
Robinson, A ;
Sarkans, U ;
Schulze-Kremer, S ;
Stewart, J ;
Taylor, R ;
Vilo, J ;
Vingron, M .
NATURE GENETICS, 2001, 29 (04) :365-371
[7]  
Buser D, 1999, J BIOMED MATER RES, V45, P75, DOI 10.1002/(SICI)1097-4636(199905)45:2<75::AID-JBM1>3.0.CO
[8]  
2-P
[9]   Evaluation of an endosseous titanium implant with a sandblasted and acid-etched surface in the canine mandible: Radiographic results [J].
Cochran, DL ;
Nummikoski, PV ;
Higginbottom, FL ;
Hermann, JS ;
Makins, SR ;
Buser, D .
CLINICAL ORAL IMPLANTS RESEARCH, 1996, 7 (03) :240-252
[10]   The scientific basis for and clinical experiences with Straumann implants including the ITI® Dental Implant System:: a consensus report [J].
Cochran, DL .
CLINICAL ORAL IMPLANTS RESEARCH, 2000, 11 :33-58