共 37 条
Chemical and enzymatic synthesis of fructose analogues as probes for import studies by the hexose transporter in parasites
被引:16
作者:
Azéma, L
Bringaud, F
Blonski, C
Périé, J
机构:
[1] Univ Toulouse 3, URA CNRS ESA 5068, Grp Chim Organ Biol, F-31062 Toulouse 4, France
[2] Univ Bordeaux 2, URA 1637, Mol Parasitol Lab, F-33076 Bordeaux, France
关键词:
D O I:
10.1016/S0968-0896(00)00018-3
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Various D-fructose analogues modified at C-l or C-6 positions were synthesized from D-glucose by taking advantage of the Amadori rearrangement or using the aldol condensation between dihydroxyacetone phosphate and appropriate aldehyde catalyzed by fructose I,6-diphosphate aldolase from rabbit muscle. Time affinities of the analogues for the glucose transporter expressed in the mammalian form of Trypanosoma brucei were determined by inhibition of radiolabelled 2-deoxy-D-glucose (2-DOG) transport using zero-trans kinetic analysis. Interestingly, the analogues bearing an aromatic group (i.e. a fluorescence marker) at C-l or C-6 positions present comparable apparent affinities to D-fructose for the transporter. This result could find applications for hexose transport studies and also provides criteria for the design of glucose import inhibitors. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:717 / 722
页数:6
相关论文