Immune responses in mice to intranasal and intracutaneous administration of a DNA vaccine encoding Helicobacter pylori-catalase

被引：39

作者：

Miyashita, M

Joh, T

Watanabe, K

Todoroki, I

Seno, K

Ohara, H

Nomura, T

Miyata, M

Kasugai, K

Tochikubo, K

Itoh, M

Niita, M

机构：

[1] Nagoya City Univ, Sch Med, Dept Internal Med 1, Mizuho Ku, Nagoya, Aichi 4678601, Japan

[2] Aichi Med Univ, Dept Internal Med 2, Aichi 4801195, Japan

[3] Nagoya City Univ, Sch Med, Dept Microbiol, Mizuho Ku, Nagoya, Aichi 4678601, Japan

来源：

VACCINE | 2002年 / 20卷 / 17-18期

关键词：

Helicobacter pylori; catalase; DNA vaccine;

D O I：

10.1016/S0264-410X(02)00104-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We previously reported that the intracutaneous injection of DNA vaccines encoding Helicobacter pylori heat shock proteins elicited 0 specific immune responses, and led to reduced infection in mice. In this study, we constructed DNA vaccine encoding H. pylori-catalase (pcDNA3.1-kat) and investigated the immune responses to intranasal and intracutaneous administration of pcDNA3.1-kat. C57/BL6 mice were immunized intracutaneously with 10 mug of pcDNA3.1-kat or intranasally with 50 mug of pcDNA3.1-kat. Catalase-specific IgG antibody was detected in the sera of intranasal and intracutaneous immunized mice. Both intranasal and intracutaneous immunized mice were significantly protected from colonization by H. pylori and had significantly reduced degrees of gastritis. These results demonstrate that DNA vaccine encoding H. pylori-catalase can induce an immune response against H. pylori, and that intranasal immunization works as well as intracutaneous immunization. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：2336 / 2342

页数：7

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