Stress-induced changes of plasma antioxidants in aquacultured sea bass, Dicentrarchus labrax

被引:51
作者
Guerriero, G [1 ]
Di Finizio, A [1 ]
Ciarcia, G [1 ]
机构
[1] Univ Naples Federico II, Dept Zool, I-80134 Naples, Italy
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 2002年 / 132卷 / 01期
关键词
alpha-tocopherol; ascorbate; glutathione peroxidase; HPLC; body mass; hypoxia; temperature; pH; salinity; chlorine; Dicentrarchus labrax;
D O I
10.1016/S1095-6433(01)00549-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Antioxidant plasma activities of ascorbate, alpha-tocopherol and glutathione peroxidase were analysed in adult male sea bass, Dicentrarchus labrax, in normal conditions and after hypoxia-recovery. In addition, tank measurements of temperature. pH. salinity and chlorine changes were carried out. Ascorbate and a-tocopherol were measured using a high-pressure liquid chromatography method and glutathione peroxidase activity enzymatically. Ascorbate and alpha-tocopherol showed a relationship with the velocity of body growing in normal and hypoxia-recovery conditions. In sea bass exposed to hypoxia. only ascorbate and alpha-tocopherol levels were significantly lower compared with the control group. Slope study and expression percent of antioxidants reduction after stress conditions revealed a predominant role of plasma alpha-tocopherol. Sea bass subjected to variations of salinity and chlorine showed a significant decrease in plasma alpha-tocopherol. A relationship could be suggested between antioxidant defence and fish response in aquaculture. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:205 / 211
页数:7
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