Short-term feeding of baicalin inhibits age-associated NF-κB activation

被引:78
作者
Kim, Dae Hyun
Kim, Hyung Keun
Park, Seongjoon
Kim, Ji Young
Zou, Yani
Cho, Ki Ho
Kim, Young Suk
Kim, Dong Hyun
Yu, Byung Pal
Choi, Jae Sue
Young-Chung, Hae [1 ]
机构
[1] Pusan Natl Univ, Coll Pharm, Dept Pharm, Pusan 609735, South Korea
[2] Kyung Hee Univ, Coll Oriental Med, Seoul 130701, South Korea
[3] Kyung Hee Univ, Coll Pharm, Seoul 130701, South Korea
[4] Univ Texas, Hlth Sci Ctr, Dept Physiol, San Antonio, TX 78229 USA
[5] Pukyong Natl Univ, Fac Food Sci & Biotechnol, Busan 608737, South Korea
关键词
NF-kappa B; aging; baicalin; oxidative stress; reactive species;
D O I
10.1016/j.mad.2006.05.002
中图分类号
Q2 [细胞生物学];
学科分类号
071009 [细胞生物学]; 090102 [作物遗传育种];
摘要
Baicalin is a favonoid isolated from Scutellaria baicalensis and is known to affect multiple biological functions, including the inhibition of aldose reductase, HIV infection, and nitric oxide producing activity. Oxidative stress is considered a major cause of aging and various age-related diseases, and among the key cellular components exquisitely sensitive to oxidative stress is the transcription factor, nuclear factor-kappa B (NF-kappa B). In the present study, we attempted to elucidate the mechanisms underlying the suppression of age-related NF-kappa B-activation by baicalin in kidney tissue from old rats. Results showed NF-kappa B activation and the upregulation of NF-kappa B targeting genes, hemoxygenase-1, inducible nitric oxide synthase (NOS), and COX-2 with age. In contrast, the increased expression of these NF-kappa B targeting genes was effectively inhibited by baicalin. Baicalin was shown to inhibit the NF-kappa B cascade via three signal transduction pathways, NIK/IKK, extracellular signal-regulated kinase (ERK), and p38 mitogen-activated protein kinase (MAPK). Our results clearly indicated the anti-oxidative effects of baicalin on age-related redox imbalance. Thus, the significance of the current study is the new information revealing the anti-oxidative properties of baicalin and the role it plays in the regulation of age-related alterations. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:719 / 725
页数:7
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