The MAP1-LC3 conjugation system is involved in lipid droplet formation

被引：224

作者：

Shibata, Masahiro ^{[2
,3
]}

Yoshimura, Kentaro ^{[2
]}

Furuya, Norihiko ^{[1
]}

Koike, Masato ^{[2
,4
]}

Ueno, Takashi ^{[1
]}

Komatsu, Masaaki ^{[1
,5
]}

Arai, Hiroyuki ^{[6
]}

Tanaka, Keiji ^{[5
]}

Kominami, Eiki ^{[1
]}

Uchiyama, Yasuo ^{[2
,4
]}

机构：

[1] Juntendo Univ, Sch Med, Dept Biochem, Bunkyo Ku, Tokyo 1138421, Japan

[2] Osaka Univ, Grad Sch Med, Dept Cell Biol & Neurosci, Suita, Osaka 5650871, Japan

[3] Niigata Univ, Grad Sch Med & Dental Sci, Dept Regenerat & Transplant Med, Div Gross Anat & Morphogenesis, Niigata 9518510, Japan

[4] Juntendo Univ, Grad Sch Med, Dept Cell Biol & Neurosci, Bunkyo Ku, Tokyo 1138421, Japan

[5] Tokyo Metropolitan Inst Med Sci, Lab Frontier Sci, Tokyo 1138613, Japan

[6] Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Hlth Chem, Bunkyo Ku, Tokyo 1130033, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 382卷 / 02期

基金：

日本学术振兴会;

关键词：

Lipid droplets; Autophagy; Autophagosomes; LC3; Triacylglycerol; Liver; Heart; Atg7; Microtubule associated protein 1A/1B light chain 3; AUTOPHAGY; PROTEIN; CELLS; MICE; BIOGENESIS; CATABOLISM; MEMBRANE; NEURONS; DISEASE; STORAGE;

D O I：

10.1016/j.bbrc.2009.03.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipid droplets (LDs) are ubiquitous in eukaryotic cells, while excess free fatty acids and glucose in plasma are converted to triacylglycerol (TAG) and stored as LDs. However, the mechanism for the generation and growth of LDs in cells is largely unknown. We show here that the LC3 lipidation system essential for macroautophagy is involved in LD formation. LD formation accompanied by accumulation of TAG induced by starvation was largely suppressed in the hepatocytes that cannot execute autophagy. Under starvation conditions, LDs in addition to autophagosomes were abundantly formed in the cytoplasm of these tissue cells. Moreover, LC3 was localized on the surface of LDs and LC3-II (lipidation form) was fractionated to a perilipin (LD marker)-positive lipid fraction from the starved liver. Taken together, these results indicate that the LC3 conjugation system is critically involved in lipid metabolism via LD formation. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：419 / 423

页数：5

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