Adsorption of plasma proteins on polyethylene oxide-modified lipid bilayers studied by total internal reflection fluorescence

被引：39

作者：

Xu, Z

Marchant, RE

机构：

[1] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Dept Macromol Sci, Cleveland, OH 44106 USA

来源：

BIOMATERIALS | 2000年 / 21卷 / 10期

关键词：

protein adsorption; polyethylene oxide conformation; steric repulsion; total internal reflection fluorescence;

D O I：

10.1016/S0142-9612(00)00004-1

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Distearoylphophatidylcholine (DSPC) mixed with various mole percentages of polyethylene oxide (number average molecular weight 2000)-grafted distearoylphosphatidylethanolamine (PEO2000-DSPE) were deposited on DSPE-coated quartz surfaces by the Langmuir-Blodgett deposition. Structural transitions in PEO2000 from pancake to mushroom, and from mushroom to brush conformations were revealed from film balance experiments. Adsorption kinetics of proteins from 1% platelet-poor plasma (PPP) on the supported lipid bilayers were studied using intrinsic total internal reflection fluorescence. FLU the supported lipid bilayers exhibited over a magnitude reduction in adsorbed plasma proteins, compared with the quartz substrate. The increase of PEO2000-DSPE density in the mixed bilayers slightly increases the amount of adsorbed proteins on the bilayers. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1075 / 1083

页数：9

共 27 条

[1]

ALEXANDER S, 1976, J PHYS FRANCE, V37, P1445

[2]

ANDRADE JD, 1996, HYDROPHILIC POLYM, P51

[3] Heteroenergetics of bovine serum albumin adsorption from good solvents related to crystallization conditions [J].