DNA extraction method for screening yeast clones by PCR

被引：54

作者：

Akada, R ^{[1
]}

Murakane, T ^{[1
]}

Nishizawa, Y ^{[1
]}

机构：

[1] Yamaguchi Univ, Fac Engn, Dept Appl Chem & Chem Engn, Ube, Yamaguchi 7558611, Japan

来源：

BIOTECHNIQUES | 2000年 / 28卷 / 04期

关键词：

D O I：

10.2144/00284st02

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple procedure for isolating yeast DNA suitable for use ns a template for PCR amplification is described. SDS treatment alone is sufficient for extraction of chromosomal DNA from yeast cells. Cells of a yeast colony are suspended in a small volume (about 20 mu L) of a 0.25% SDS solution, mixed vigorously and centrifuged. The supernatant cart be directly used as a template after dilution to give nn SDS concentration of less than 0.01% in the final PCR mixture.

引用

页码：668 / +

页数：4

共 13 条

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