Synthesis of RNA using 2′-O-DTM protection

被引：64

作者：

Semenyuk, Andrey

Foldesi, Andras

Johansson, Tommy

Estmer-Nilsson, Camilla

Blomgren, Peter

Brannvall, Mathias

Kirsebom, Leif A.

Kwiatkowski, Marek ^{[1
]}

机构：

[1] Uppsala Univ, Dept Genet & Pathol, Uppsala, Sweden

[2] Quiatech AB, Uppsala, Sweden

[3] Uppsala Univ, BMC, Dept Cell & Mol Biol, Uppsala, Sweden

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 38期

关键词：

D O I：

10.1021/ja0636587

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

tert-Butyldithiomethyl (DTM), a novel hydroxyl protecting group, cleavable under reductive conditions, was developed and applied for the protection of 2′-OH during solid-phase RNA synthesis. This function is compatible with all standard protecting groups used in oligonucleotide synthesis, and allows for fast and high-yield synthesis of RNA. Oligonucleotides containing the 2′-O-DTM groups can be easily deprotected under the mildest possible aqueous and homogeneous conditions. The preserved 5′-O-DMTr function can be used for high-throughput cartridge RNA purification. Copyright © 2006 American Chemical Society.

引用

页码：12356 / 12357

页数：2

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