A novel Arthrobacter beta-galactosidase with homology to eucaryotic beta-galactosidases

被引:22
作者
Gutshall, K [1 ]
Wang, K [1 ]
Brenchley, JE [1 ]
机构
[1] PENN STATE UNIV,DEPT BIOCHEM & MOL BIOL,UNIVERSITY PK,PA 16802
关键词
D O I
10.1128/jb.179.9.3064-3067.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An Arthrobacter beta-galactosidase has homolog with the lysosomal acid beta-galactosidases from humans and mice and with a Xanthomonas manihotis enzyme, Phylogenetic analysis of the deduced amino acid sequence showed an unusual pattern, with this procaryotic enzyme clustering within the animal clade, The gene encodes a subunit of 52 kDa, and the enzyme appears to be active as a dimer. The enzyme hydrolyzed substrates with either a beta-1,4 or a beta-1,3 linkage.
引用
收藏
页码:3064 / 3067
页数:4
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