Normal growth and differentiation in a spontaneously immortalized near-diploid human keratinocyte cell line, NIKS

被引:188
作者
Allen-Hoffmann, BL
Schlosser, SJ
Ivarie, CAR
Sattler, CA
Meisner, LF
O'Connor, SL
机构
[1] Univ Wisconsin, Sch Med, Dept Pathol, Madison, WI 53706 USA
[2] Univ Wisconsin, Sch Med, McArdle Lab Canc Res, Madison, WI 53706 USA
关键词
hemidesmosomes; nucleosomal fragmentation; organotypic culture; trisomy;
D O I
10.1046/j.1523-1747.2000.00869.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
We report the isolation and characterization of a spontaneously immortalized human keratinocyte cell line, NIKS. The cell line is not tumorigenic in athymic nude mice and maintains cell-type-specific requirements for growth in vitro. NIKS cells express steady-state levels of transforming growth factor-alpha, transforming growth factor-beta 1, epidermal growth factor receptor, c-myc, and keratin 14 mRNAs comparable with the parental BC-1-Ep keratinocyte strain. BC-1-Ep and NIKS keratinocytes produce similar levels of cornified envelopes and nucleosomal fragmentation in response to loss of substrata attachment. DNA fingerprinting results confirm that the NIKS cells originated from the parental BC-1-Ep keratinocytes. NIKS cells contain 47 chromosomes due to an extra isochromosome of the long arm of chromosome 8, and the near-diploid karyotype appears to be stable with repeated passage. A fully stratified squamous epithelium is formed by the NIKS keratinocytes in organotypic culture. Ultrastructural analysis of both the parental and immortalized keratinocytes reveals abundant desmosomes, hemidesmosomes, and the production of a basal lamina. Our findings with the NIKS cells support the observation that spontaneous immortalization is not linked to alterations in squamous differentiation or the ability to undergo apoptosis. The NIKS human keratinocyte cell line is an important new tool for the study of growth and differentiation in stratified squamous epithelia.
引用
收藏
页码:444 / 455
页数:12
相关论文
共 61 条
[1]   POLYCYCLIC AROMATIC HYDROCARBON MUTAGENESIS OF HUMAN EPIDERMAL-KERATINOCYTES IN CULTURE [J].
ALLENHOFFMANN, BL ;
RHEINWALD, JG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (24) :7802-7806
[2]  
ALLENHOFFMANN BL, 1990, J BIOL CHEM, V265, P5219
[3]  
BADEN HP, 1987, IN VITRO CELL DEV B, V23, P205
[4]   REGULATION OF EPITHELIAL-CELL PROLIFERATION BY TRANSFORMING GROWTH-FACTORS [J].
BASCOM, CC ;
SIPES, NJ ;
COFFEY, RJ ;
MOSES, HL .
JOURNAL OF CELLULAR BIOCHEMISTRY, 1989, 39 (01) :25-32
[5]   IS P53 POLYMORPHISM MAINTAINED BY NATURAL-SELECTION [J].
BECKMAN, G ;
BIRGANDER, R ;
SJALANDER, A ;
SAHA, N ;
HOLMBERG, PA ;
KIVELA, A ;
BECKMAN, L .
HUMAN HEREDITY, 1994, 44 (05) :266-270
[6]   PRODUCTION OF A TISSUE-LIKE STRUCTURE BY CONTRACTION OF COLLAGEN LATTICES BY HUMAN-FIBROBLASTS OF DIFFERENT PROLIFERATIVE POTENTIAL INVITRO [J].
BELL, E ;
IVARSSON, B ;
MERRILL, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (03) :1274-1278
[7]   Reconstruction of a human skin equivalent using a spontaneously transformed keratinocyte cell line (HaCaT) [J].
Boelsma, E ;
Verhoeven, MCH ;
Ponec, M .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1999, 112 (04) :489-498
[8]   NORMAL KERATINIZATION IN A SPONTANEOUSLY IMMORTALIZED ANEUPLOID HUMAN KERATINOCYTE CELL-LINE [J].
BOUKAMP, P ;
PETRUSSEVSKA, RT ;
BREITKREUTZ, D ;
HORNUNG, J ;
MARKHAM, A ;
FUSENIG, NE .
JOURNAL OF CELL BIOLOGY, 1988, 106 (03) :761-771
[9]   Epidermal differentiation and basement membrane formation by HaCaT cells in surface transplants [J].
Breitkreutz, D ;
Schoop, VM ;
Mirancea, N ;
Baur, M ;
Stark, HJ ;
Fusenig, NE .
EUROPEAN JOURNAL OF CELL BIOLOGY, 1998, 75 (03) :273-286
[10]   CULTURING KERATINOCYTES AND FIBROBLASTS IN A 3-DIMENSIONAL MESH RESULTS IN EPIDERMAL DIFFERENTIATION AND FORMATION OF A BASAL LAMINA-ANCHORING ZONE [J].
CONTARD, P ;
BARTEL, RL ;
JACOBS, L ;
PERLISH, JS ;
MACDONALD, ED ;
HANDLER, L ;
CONE, D ;
FLEISCHMAJER, R .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1993, 100 (01) :35-39