Aberrant mRNA processing of the maize Rp1-D rust resistance gene in wheat and barley

被引:21
作者
Ayliffe, MA
Steinau, M
Park, RF
Rooke, L
Pacheco, MG
Hulbert, SH
Trick, HN
Pryor, AJ
机构
[1] CSIRO, Plant Ind, Canberra, ACT 2601, Australia
[2] Kansas State Univ, Throckmorton Plant Sci Ctr, Dept Plant Pathol, Manhattan, KS 66505 USA
[3] Univ Sydney, Plant Breeding Inst Cobb, Camden, NSW 2570, Australia
关键词
cereal; R gene;
D O I
10.1094/MPMI.2004.17.8.853
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The maize Rp1-D gene confers race-specific resistance against Puccinia sorghi (common leaf rust) isolates containing a corresponding avrRp1-D avirulence gene. An Rp1-D genomic clone and a similar Rp1-D transgene regulated by the maize ubiquitin promoter were transformed independently into susceptible maize lines and shown to confer Rp1-D resistance, demonstrating that this resistance can be transferred as a single gene. Transfer of these functional transgenes into wheat and barley did not result in novel resistances when these plants were challenged with isolates of wheat stem rust (P. graminis), wheat leaf rust (P. triticina), or barley leaf rust (P. hordei). Regardless of the promoter employed, low levels of gene expression were observed. When constitutive promoters were used for transgene expression, a majority of Rp1-D transcripts were truncated in the nucleotide binding site-encoding region by premature polyadenylation. This aberrant mRNA processing was unrelated to gene function because an inactive version of the gene also generated such transcripts. These data demonstrate that resistance gene transfer between species may not be limited only by divergence of signaling effector molecules and pathogen avirulence ligands, but potentially also by more fundamental gene expression and transcript processing limitations.
引用
收藏
页码:853 / 864
页数:12
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