The Drosophila BRM complex facilitates global transcription by RNA polymerase II

被引:133
作者
Armstrong, JA
Papoulas, O
Daubresse, G
Sperling, AS
Lis, JT
Scott, MP
Tamkun, JW [1 ]
机构
[1] Univ Calif Santa Cruz, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA
[2] Stanford Univ, Howard Hughes Med Inst, Dept Dev Biol, Sch Med, Stanford, CA 94305 USA
[3] Stanford Univ, Howard Hughes Med Inst, Dept Genet, Sch Med, Stanford, CA 94305 USA
[4] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA
关键词
Brahma; hromatin remodeling; Polycomb; trithorax group;
D O I
10.1093/emboj/cdf517
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Drosophila brahma (brm) encodes the ATPase subunit of a 2 MDa complex that is related to yeast SWI/SNF and other chromatin-remodeling complexes. BRM was identified as a transcriptional activator of Hox genes required for the specification of body segment identities. To clarify the role of the BRM complex in the transcription of other genes, we examined its distribution on larval salivary gland polytene chromosomes. The BRM complex is associated with nearly all transcriptionally active chromatin in a pattern that is generally non-overlapping with that of Polycomb, a repressor of Hox gene transcription. Reduction of BRM function dramatically reduces the association of RNA polymerase II with salivary gland chromosomes. A few genes, such as induced heat shock loci, are not associated with the BRM complex; transcription of these genes is not compromised by loss of BRM function. The distribution of the BRM complex thus correlates with a dependence on BRM for gene activity. These data suggest that the chromatin remodeling activity of the BRM complex plays a general role in facilitating transcription by RNA polymerase Il.
引用
收藏
页码:5245 / 5254
页数:10
相关论文
共 70 条
  • [1] High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo:: roles in promoter proximal pausing and transcription elongation
    Andrulis, ED
    Guzmán, E
    Döring, P
    Werner, J
    Lis, JT
    [J]. GENES & DEVELOPMENT, 2000, 14 (20) : 2635 - 2649
  • [2] Preferential interaction of the core histone tail domains with linker DNA
    Angelov, D
    Vitolo, JM
    Mutskov, V
    Dimitrov, S
    Hayes, JJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (12) : 6599 - 6604
  • [3] A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro
    Armstrong, JA
    Bieker, JJ
    Emerson, BM
    [J]. CELL, 1998, 95 (01) : 93 - 104
  • [4] The chromatin remodelling factor Brg-1 interacts with β-catenin to promote target gene activation
    Barker, N
    Hurlstone, A
    Musisi, H
    Miles, A
    Bienz, M
    Clevers, H
    [J]. EMBO JOURNAL, 2001, 20 (17) : 4935 - 4943
  • [5] Boyer LA, 2000, BIOESSAYS, V22, P666, DOI 10.1002/1521-1878(200007)22:7<666::AID-BIES9>3.3.CO
  • [6] 2-P
  • [7] BRIZUELA BJ, 1994, GENETICS, V137, P803
  • [8] A Brg1 null mutation in the mouse reveals functional differences among mammalian SWI/SNF complexes
    Bultman, S
    Gebuhr, T
    Yee, D
    La Mantia, C
    Nicholson, J
    Gilliam, A
    Randazzo, F
    Metzger, D
    Chambon, P
    Crabtree, G
    Magnuson, T
    [J]. MOLECULAR CELL, 2000, 6 (06) : 1287 - 1295
  • [9] RSC, an essential, abundant chromatin-remodeling complex
    Cairns, BR
    Lorch, Y
    Li, Y
    Zhang, MC
    Lacomis, L
    ErdjumentBromage, H
    Tempst, P
    Du, J
    Laurent, B
    Kornberg, RD
    [J]. CELL, 1996, 87 (07) : 1249 - 1260
  • [10] Osa associates with the Brahma chromatin remodeling complex and promotes the activation of some target genes
    Collins, RT
    Furukawa, T
    Tanese, N
    Treisman, JE
    [J]. EMBO JOURNAL, 1999, 18 (24) : 7029 - 7040