Nanostructure Initiator Mass Spectrometry: Tissue Imaging and Direct Biofluid Analysis

被引：93

作者：

Yanes, Oscar ^{[1
,3
]}

Woo, Hin-Koon ^{[1
,3
]}

Northen, Trent R. ^{[1
,3
]}

Oppenheimer, Stacey R. ^{[2
]}

Shriver, Leah ^{[4
]}

Apon, Jon ^{[1
,3
]}

Estrada, Mayra N. ^{[4
]}

Potchoiba, Michael J. ^{[2
]}

Steenwyk, Rick ^{[2
]}

Manchester, Marianne ^{[4
]}

Siuzdak, Gary ^{[1
,3
]}

机构：

[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[2] Pfizer Global Res & Dev, Dept Pharmacokinet Dynam & Metab, Groton, CT 06340 USA

[3] Scripps Res Inst, Scripps Ctr Mass Spectrometry, La Jolla, CA 92037 USA

[4] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 08期

基金：

美国国家卫生研究院;

关键词：

ATMOSPHERIC-PRESSURE; HIGH-SENSITIVITY; SIMS; MS; METHODOLOGY; METABOLITES; PROTEINS; URINE; DRUGS; RAT;

D O I：

10.1021/ac802576q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nanostructure initiator mass spectrometry (NIMS) is a recently introduced matrix-free desorption/ionization platform that requires minimal sample preparation. Its application to xenobiotics and endogenous metabolites in tissues is demonstrated, where clozapine and N-desmethylclozapine were observed from mouse and rat brain sections. It has also been applied to direct biofluid analysis where ketamine and norketamine were observed from plasma and urine. Detection of xenobiotics from biofluids was made even more effective using a novel NIMS on-surface extraction method taking advantage of the hydrophobic nature of the initiator. Linear response and limit of detection were also evaluated for xenobiotics such as methamphetamine, codeine, alprazolam, and morphine, revealing that NIMS can be used for quantitative analysis. Overall, our results demonstrate the capacity of NIMS to perform sensitive, simple, and rapid analyses from highly complex biological tissues and fluids.

引用

页码：2969 / 2975

页数：7

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