Expression of the calcium-independent cytokine-inducible (iNOS) isoform of nitric oxide synthase in rat placenta

被引：16

作者：

Casado, M

DiazGuerra, MJM

Rodrigo, J

Fernandez, AP

Bosca, L

MartinSanz, P

机构：

[1] UNIV COMPLUTENSE MADRID, FAC FARM, CSIC, INST BIOQUIM, E-28040 MADRID, SPAIN

[2] INST NEUROBIOL SANTIAGO RAMON Y CAJAL, CSIC, MADRID 28071, SPAIN

来源：

BIOCHEMICAL JOURNAL | 1997年 / 324卷

关键词：

D O I：

10.1042/bj3240201

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of the calcium-independent cytokine-inducible nitric oxide synthase (iNOS) has been investigated in rat placenta from day 19 of gestation till delivery. iNOS has been detected at the mRNA, enzyme activity and protein levels in complete placenta. Immunocytochemical detection of iNOS was heterogeneously distributed in control placenta. Intraperitoneal injection of pregnant rats at 21 days of gestation with lipopolysaccharide (LPS) increased the iNOS immunoreactivity in the decidua basalis of the placenta, and, when the mRNA levels and enzyme activity were measured in total tissue, a moderate increase (approx. 160%) was observed. A constitutive nuclear factor kappa B activity was observed in placenta from both control and LPS-treated animals. These results indicate constitutive expression of iNOS in rat placenta.

引用

页码：201 / 207

页数：7

共 44 条

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