Priming with cold-adapted influenza A does not prevent infection but elicits long-lived protection against supralethal challenge with heterosubtypic virus

被引：119

作者：

Powell, Timothy J. ^{[1
]}

Strutt, Tara ^{[1
]}

Reome, Joyce ^{[1
]}

Hollenbaugh, Joseph A. ^{[1
]}

Roberts, Alan D. ^{[1
]}

Woodland, David L. ^{[1
]}

Swain, Susan L. ^{[1
]}

Dutton, Richard W. ^{[1
]}

机构：

[1] Trudeau Inst Inc, Saranac Lake, NY 12983 USA

来源：

JOURNAL OF IMMUNOLOGY | 2007年 / 178卷 / 02期

关键词：

D O I：

10.4049/jimmunol.178.2.1030

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We show in this study several novel features of T cell-based heterosubtypic immunity against the influenza A virus in mice. First, T cell-mediated heterosubtypic protection against lethal challenge can be generated by a very low priming dose. Second, it becomes effective within 5-6 days. Third, it provides protection against a very high dose challenge for > 70 days. Also novel is the finding that strong, long-lasting, heterosubtypic protection can be elicited by priming with attenuated cold-adapted strains. We demonstrate that priming does not prevent infection of the lungs following challenge, but leads to earlier clearance of the virus and 100% survival after otherwise lethal challenge. Protection is dependent on CD8 T cells, and we show that CD4 and CD8 T cells reactive to conserved epitopes of the core proteins of the challenge virus are present after priming. Our results suggest that intranasal vaccination with cold-adapted, attenuated live virus has the potential to provide effective emergency protection against emerging influenza strains for several months.

引用

页码：1030 / 1038

页数：9

共 21 条

[1] Heterosubtypic immunity to influenza A virus in mice lacking IgA, all Ig, NKT cells, or γδ T cells [J].