Identification of macrophage migration inhibitory factor and human neutrophil peptides 1-3 as potential biomarkers for gastric cancer

被引：43

作者：

Mohri, Y. ^{[1
,2
]}

Mohri, T. ^{[1
,3
]}

Wei, W. ^{[1
]}

Qi, Y-J ^{[1
]}

Martin, A. ^{[1
]}

Miki, C. ^{[4
]}

Kusunoki, M. ^{[4
]}

Ward, D. G. ^{[1
]}

Johnson, P. J. ^{[1
]}

机构：

[1] Univ Birmingham, Canc Res UK, Inst Canc Studies, Sch Canc Sci, Birmingham B15 2TT, W Midlands, England

[2] Mie Univ, Dept Innovat Surg, Grad Sch Med, Tsu, Mie 5148507, Japan

[3] Toyama Hosp, Dept Surg, Tsu, Mie 5140043, Japan

[4] Mie Univ, Dept Gastrointestinal & Pediat Surg, Grad Sch Med, Tsu, Mie 5148507, Japan

来源：

BRITISH JOURNAL OF CANCER | 2009年 / 101卷 / 02期

关键词：

gastric; biomarker; serum; proteome; ALPHA-DEFENSINS; CARCINOEMBRYONIC ANTIGEN; FACTOR MIF; CA; 72-4; CA-19-9; OVEREXPRESSION; STATISTICS; CARCINOMAS; EXPRESSION; DIAGNOSIS;

D O I：

10.1038/sj.bjc.6605138

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

BACKGROUND: Proteomic methods have the potential to meet the urgent need for better cancer biomarkers. We have used a range of proteomic analyses of serum and tissue from gastric cancer patients and relevant controls to discover biomarkers for gastric cancer. METHODS: Surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI) and antibody arrays were used to compare protein expression in 21 pairs of gastric cancer tissue and adjacent normal mucosa and serum from 51 gastric cancer patients and 29 patients with benign gastric diseases. Expression differences were confirmed by enzyme-linked immunosorbent assay. RESULTS: Tissue analysis shows human neutrophil peptides 1-3 (HNPs 1-3) elevated 10-fold (P = 0.001) in gastric cancer relative to adjacent normal mucosa. Macrophage migration inhibitory factor (MIF) was increased five-fold (P = 1.84 x 10(-7)) in the serum of gastric cancer patients relative to individuals with benign gastric disease. The large increase in MIF concentration in serum gives an area under the receiver operating characteristic curve of 0.85. CONCLUSIONS: Proteomic analyses of serum and tissue indicate that HNPs 1-3 and MIF have potential as biomarkers for gastric cancer. In particular MIF may be useful, either alone or in combination with other markers, for diagnosing and monitoring gastric cancer. British Journal of Cancer (2009) 101, 295-302. doi:10.1038/sj.bjc.6605138 www.bjcancer.com Published online 23 June 2009 (C) 2009 Cancer Research UK

引用

页码：295 / 302

页数：8

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