Evaluation of spectral imaging for plant cell analysis

被引：46

作者：

Berg, RH ^{[1
]}

机构：

[1] Donald Danforth Plant Sci Ctr, Integrated Microscopy Facil, St Louis, MO 63132 USA

来源：

JOURNAL OF MICROSCOPY-OXFORD | 2004年 / 214卷

关键词：

autofluorescence; confocal microscopy; multiphoton microscopy; spectral imaging; unmixing;

D O I：

10.1111/j.0022-2720.2004.01347.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Fluorescence imaging at high spectral resolution is now a practical reality and has great promise in plant cell biology. Emission spectral curve data can be used computationally to distinguish spectrally similar fluorophores, or to remove autofluorescence, and to spectrally analyse autofluorescent molecules, which are especially abundant in plant tissues. Examples of these applications in plant cells are given, and a comparison is made between the current offerings in spectral imaging laser scanning confocal microscopes.

引用

页码：174 / 181

页数：8

共 13 条

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