The generation of proper constitutive G-tails on yeast telomeres is dependent on the MRX complex

被引：193

作者：

Larrivée, M ^{[1
]}

LeBel, C ^{[1
]}

Wellinger, RJ ^{[1
]}

机构：

[1] Univ Sherbrooke, Fac Med, Dept Microbiol & Infectol, Grp ARN RNA, Sherbrooke, PQ J1H 5N4, Canada

来源：

GENES & DEVELOPMENT | 2004年 / 18卷 / 12期

关键词：

telomeres; G-tails; MRX complex; yeast;

D O I：

10.1101/gad.1199404

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The precise DNA arrangement at chromosomal ends and the proteins involved in its maintenance are of crucial importance for genome stability. For the yeast Saccharomyces cerevisiae, this constitutive DNA configuration has remained unknown. We demonstrate here that G-tails of 12-14 bases are present outside of S phase on normal yeast telomeres. Furthermore, the Mre11p protein is essential for the proper establishment of this constitutive end-structure. However, the timing of extended G-tails occurring during S phase is not affected in strains lacking Mre11p. Thus, G-tails are present on yeast chromosomes throughout the cell cycle and the MRX complex is required for their normal establishment.

引用

页码：1391 / 1396

页数：6

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