Photoreactivation of Legionella pneumophila after inactivation by low- or medium-pressure ultraviolet lamp

被引：83

作者：

Oguma, K

Katayama, H

Ohgaki, S

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Urban Engn, Bunkyo Ku, Tokyo 1138656, Japan

[2] Univ Tokyo, Grad Sch Frontier Sci, Inst Environm Studies, Bunkyo Ku, Tokyo 1138656, Japan

来源：

WATER RESEARCH | 2004年 / 38卷 / 11期

关键词：

Escherichia coli; Legionella pneumophila; low-pressure UV lamp; medium-pressure UV lamp; photoreactivation; ultraviolet disinfection;

D O I：

10.1016/j.watres.2004.03.024

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Photoreactivation of Legionella pneumophila after the inactivation by low-pressure (LP) or medium-pressure (NIP) UV lamp was investigated in comparison with that of Escherichia coli. An endonuclease sensitive site (ESS) assay was used to determine the number of UV-induced pyrimidine dimers in the genome DNA of L. pneumophila or E. coli, while the survival ratio of each bacterium was also investigated by cultivation methods. L. pneumophila performed photoreactivation with almost complete repair of pyrimidine dimers associated with the quick recovery of survival ratio. A 3 log inactivation of L. pneumophila by LP or MP UV lamp was, respectively, resulted in 0.5 log or 0.4 log inactivation when photoreactivation was completed. Interestingly, L. pneumophila performed equivalent photoreactivation after LP and NIP UV lamp exposures while photoreactivation of E coli was significantly repressed after the inactivation by MP UV lamp. This study indicated that an attention would be required to design and operate a UV disinfection system targeting L. pneumophila. It was further implied that E coli would not correctly indicate the fate of L. pneumophila in UV disinfection systems when photoreactivation takes place. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：2757 / 2763

页数：7

共 30 条

[1]

[Anonymous], BIOL EFFECTS ULTRAVI

[2] Legionella:: from environmental habitats to disease pathology, detection and control [J].