Rapid and sensitive detection of D-hydantoinase producing microorganisms by using microtiter plate assay

被引：7

作者：

Chien, HCR

Hsu, WH

机构：

[1] CHUNG SHAN MED & DENT COLL,DEPT MICROBIOL,TAICHUNG,TAIWAN

[2] NATL CHUNGHSING UNIV,INST MOL BIOL,TAICHUNG,TAIWAN

来源：

BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 11期

关键词：

D O I：

10.1007/BF00154677

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

D-Hydantoinase activity of microorganisms was detected by a microtiter plate assay using dihydrouracil as substrate in 24 h. The detection gave a clear result compared to the spot-test in which the colour development of one colony interfered with colour development by adjacent colonies. The sensitivity of the detection was also higher than that of the spot-test.

引用

页码：879 / 882

页数：4

共 9 条

[1]

CHEVALIER P, 1989, APPL MICROBIOL BIOT, V30, P482

[2] MOLECULAR-CLONING OF THE PLASMID RP4 PRIMASE REGION IN A MULTI-HOST-RANGE TACP EXPRESSION VECTOR [J].