Molecular cloning and gene expression of Foxl2 in the Nile tilapia, Oreochromis niloticus

被引:122
作者
Wang, DS
Kobayashi, T
Zhou, LY
Nagahama, Y [1 ]
机构
[1] Natl Inst Basic Biol, Reprod Biol Lab, Okazaki 4448585, Japan
[2] CREST, Kawaguchi, Saitama 3320012, Japan
[3] SW China Normal Univ, Fac Life Sci, Chongqing 400715, Peoples R China
[4] Grad Univ Adv Studies, Dept Mol Biomech, Okazaki, Aichi 4448585, Japan
[5] Natl Res Inst Aquaculture, Tamaki, Mie 51604233, Japan
关键词
Foxl2; cDNA cloning; gene expression; sexual dimorphic pattern; RT-PCR; northern blot; in situ hybridization; Nile tilapia;
D O I
10.1016/j.bbrc.2004.05.133
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A Fox12 cDNA was cloned from the Nile tilapia ovary by RT-PCR and subsequent RACE. Alignment of known Fox12 sequences from vertebrates confirmed the conservation of the Fox12 open reading frame and protein sequences, especially the forkhead domain and C-terminal region, while some homopolymeric runs of amino acids are found only in mammals but not in non-mammalian vertebrates. RT-PCR revealed that Fox12 is expressed in the tilapia brain (B), pituitary (P), gill, and gonads (G), with the highest level of expression in the ovary, reflecting the involvement of Fox12 in B-P-G axis. Northern blotting and in situ hybridization also revealed an evident sexual dimorphic expression pattern in the gonads. Fox12 mRNA was mainly detected in the granulosa cells surrounding the oocytes. The ovarian expression of Fox12 in tilapia begins early during the differentiation of the gonads and persists until adulthood, implying the involvement of Fox12 in fish gonad differentiation and the maintenance of ovarian function. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:83 / 89
页数:7
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