STEAP: A prostate-specific cell-surface antigen highly expressed in human prostate tumors

被引:274
作者
Hubert, RS [1 ]
Vivanco, I [1 ]
Chen, E [1 ]
Rastegar, S [1 ]
Leong, K [1 ]
Mitchell, SC [1 ]
Madraswala, R [1 ]
Zhou, YH [1 ]
Kuo, J [1 ]
Raitano, AB [1 ]
Jakobovits, A [1 ]
Saffran, DC [1 ]
Afar, DEH [1 ]
机构
[1] UroGenesys Inc, Santa Monica, CA 90404 USA
关键词
cancer; six-transmembrane-domain protein; epithelial marker;
D O I
10.1073/pnas.96.25.14523
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In search of novel genes expressed in metastatic prostate cancer, we subtracted cDNA isolated from benign prostatic hypertrophic tissue from cDNA isolated from a prostate cancer xenograft model that mimics advanced disease. One novel gene that is highly expressed in advanced prostate cancer encodes a 339-amino acid protein with six potential membrane-spanning regions flanked by hydrophilic amino- and carboxyl-terminal domains. This structure suggests a potential function as a channel or transporter protein. This gene, named STEAP for six-transmembrane epithelial antigen of the prostate, is expressed predominantly in human prostate tissue and is up-regulated in multiple cancer cell lines, including prostate, bladder, colon, ovarian, and Ewing sarcoma. Immunohistochemical analysis of clinical specimens demonstrates significant STEAP expression at the cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. Little to no staining was detected at the plasma membranes of normal, nonprostate human tissues, except for bladder tissue, which expressed low levels of STEAP at the cell membrane. Protein analysis located STEAP at the cell surface of prostate-cancer cell lines. Our results support STEAP as a cell-surface tumor-antigen target for prostate cancer therapy and diagnostic imaging.
引用
收藏
页码:14523 / 14528
页数:6
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