Preliminary studies on anti-tumor activity of 2′,4′-dihydroxychalcone isolated from Herba Oxytropis in human gastric cancer MGC-803 cells

被引：45

作者：

Lou, Chenghua ^{[1
]}

Wang, Mingyan ^{[1
]}

Yang, Guangming ^{[1
]}

Cai, Hao ^{[1
]}

Li, Yu ^{[1
]}

Zhao, Fengming ^{[1
]}

Yang, Huan ^{[1
]}

Tong, Li ^{[2
]}

Cai, Baochang ^{[1
]}

机构：

[1] Nanjing Univ Chinese Med, Jiangsu Key Lab Chinese Med Proc, Nanjing 210029, Peoples R China

[2] Qinghai Univ, Coll Med, Xining 810001, Peoples R China

来源：

TOXICOLOGY IN VITRO | 2009年 / 23卷 / 05期

关键词：

Tibetan medicine; Herba Oxytropis; 2; 4; '-Dihydroxychalcone; Apoptosis; MGC-803; cells; ANGELICA-KEISKEI; APOPTOSIS; FLAVONOIDS; ACTIVATION; CHALCONE; APIGENIN; PATHWAY; ARREST; INDUCTION; GROWTH;

D O I：

10.1016/j.tiv.2009.05.002

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

2',4'-Dihydroxychalcone (TFC), one of the main components in Herba Oxytropis, belongs to the flavonoid group, which is known to have anti-tumor activity in vitro. In this study, the authors examined the effects of TFC on cell proliferation and apoptosis in human gastric cancer MGC-803 cells. The MTT assay results showed that TFC was able to induce cytotoxicity in MGC-803 cells in a concentration- and time-dependent manner. Acridine orange/ethidium bromide (AO/EB) staining analysis indicated that the cytotoxicity induced by TFC was mediated by apoptosis, and flow cytometry analysis indicated an increase in apoptotic cells after treatment with TFC. Furthermore, typical apoptotic morphology such as condensed chromatin, irregular nuclei. vacuoles, and dispersed granular material in the nuclear compartment were also observed using a transmission electron microscope. These results suggested that TFC can inhibit the growth of MGC-803 cells and induce apoptosis. However, further studies are necessary to investigate the possible mechanism. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：906 / 910

页数：5

共 37 条

[1]

Chalcones, coumarins, and flavanones from the exudate of Angelica keiskei and their chemopreventive effects [J].