The solution structure of the invasive tip complex from Afa/Dr fibrils

被引：38

作者：

Cota, Ernesto

Jones, Celine

Simpson, Peter

Altroff, Harri

Anderson, Kirstine L.

du Merle, Laurence

Guignot, Julie

Servin, Alain

Le Bouguenec, Chantal

Mardon, Helen

Matthews, Stephen

机构：

[1] Imperial Coll London, Div Mol Biosci, London SW7 2AZ, England

[2] Univ Oxford, Womens Ctr, John Radcliffe Hosp, Nuffield Dept Obstet & Gynaecol,Div Med Sci, Oxford OX3 9DU, England

[3] Inst Pasteur, Unite Pathogenie Bacterienne Muqueuses, F-75724 Paris 15, France

[4] Fac Pharm Paris 11, INSERM, Unite 510, Chatenay Malabry, France

来源：

MOLECULAR MICROBIOLOGY | 2006年 / 62卷 / 02期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1111/j.1365-2958.2006.05375.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Afa/Dr family of adhesins are produced by pathogenic Escherichia coli strains that are especially prevalent in chronic diarrhoeal and recurrent urinary tract infections. Most notably, they are found in up to 50% of cystitis cases in children and 30% of pyelonephritis in pregnant women. Afa/Dr adhesins are capped surface fibrils that mediate recognition of the host and subsequent bacterial internalization. Using the newly solved three-dimensional structure of the minimal invasive complex (AfaDE) combined with biochemical and cellular assays, we reveal the architecture of the fibrillar cap and identify a novel mode of synergistic integrin recognition.

引用

收藏

页码：356 / 366

页数：11

相关论文

共 41 条

[1] The eighth FIII domain of human fibronectin promotes integrin α5β1 binding via stabilization of the ninth FIII domain [J].

Altroff, H ;

van der Walle, CF ;

Asselin, J ;

Fairless, R ;

Campbell, ID ;

Mardon, HJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (42) :38885-38892

[2] Interdomain tilt angle determines integrin-dependent function of the ninth and tenth FIII domains of human fibronectin [J].

Altroff, H ;

Schlinkert, R ;

van der Walle, CF ;

Bernini, A ;

Campbell, ID ;

Werner, JM ;

Mardon, HJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (53) :55995-56003

[3] An atomic resolution model for assembly, architecture, and function of the Dr adhesins [J].

Anderson, KL ;

Billington, J ;

Pettigrew, D ;

Cota, E ;

Simpson, P ;

Roversi, P ;

Chen, HA ;

Urvil, P ;

du Merle, L ;

Barlow, PN ;

Medof, ME ;

Smith, RAG ;

Nowicki, B ;

Le Bouguénec, C ;

Lea, SM ;

Matthews, S .

MOLECULAR CELL, 2004, 15 (04) :647-657

[4] Letter to the Editor:: Complete resonance assignments of a 'donor-strand complemented' AfaE:: The afimbrial adhesin from Diffusely Adherent E-coli [J].

Anderson, KL ;

Cota, E ;

Simpson, P ;

Chen, HA ;

du Merle, L ;

Le Bouguénec, C ;

Matthews, S .

JOURNAL OF BIOMOLECULAR NMR, 2004, 29 (03) :409-410

[5] Differential recognition of members of the carcinoembryonic antigen family by Afa/Dr adhesins of diffusely adhering Escherichia coli (Afa/Dr DAEC) [J].

Berger, CN ;

Billker, O ;

Meyer, TF ;

Servin, AL ;

Kansau, I .

MOLECULAR MICROBIOLOGY, 2004, 52 (04) :963-983

[6] Bacterial pathogenesis: exploiting cellular adherence [J].

Boyle, EC ;

Finlay, BB .

CURRENT OPINION IN CELL BIOLOGY, 2003, 15 (05) :633-639

[7] ENGINEERED DISULFIDE BONDS AS PROBES OF THE FOLDING PATHWAY OF BARNASE - INCREASING THE STABILITY OF PROTEINS AGAINST THE RATE OF DENATURATION [J].

CLARKE, J ;

FERSHT, AR .

BIOCHEMISTRY, 1993, 32 (16) :4322-4329

[8]

Copié V, 1998, J MOL BIOL, V277, P663

[9] Letter to the Editor:: Complete resonance assignments of the 'donor-strand complemented' AfaD:: The afimbrial invasin from Diffusely Adherent E-coli [J].

Cota, E ;

Chen, HA ;

Anderson, KL ;

Simpson, P ;

du Merle, L ;

Bernier-Fébreau, C ;

Piaatek, R ;

Zalewska, B ;

Nowicki, B ;

Kur, J ;

Le Bouguénec, C ;

Matthews, S .

JOURNAL OF BIOMOLECULAR NMR, 2004, 29 (03) :411-412

[10]

Cota E, 2000, PROTEIN SCI, V9, P112

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