Developmental changes in the expression of enterocytic and hepatic cytochromes P4501A in rat

被引：11

作者：

Johnson, TN ^{[1
]}

Tanner, MS

Tucker, GT

机构：

[1] Univ Sheffield, Royal Hallamshire Hosp, Div Clin Sci, Acad Unit Mol Pharmacol & Pharmacogenet, Sheffield S10 2JF, S Yorkshire, England

[2] Univ Sheffield, Childrens Hosp, Acad Unit Child Hlth, Sheffield S10 2TH, S Yorkshire, England

来源：

XENOBIOTICA | 2002年 / 32卷 / 07期

关键词：

D O I：

10.1080/00498250210131888

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. The development of CYP1A enzymes was studied in enterocytic and hepatic microsomes from 1-day-old to adult male and female rats. Microsomes were prepared by calcium precipitation. Enzyme expression was determined by Western blotting using a polyclonal CYP1A1 antibody. 2. The developmental expression of CYP1A in enterocytic and hepatic microsomes was similar for males and females. 3. Enterocytic CYP1A (CYP1A1) showed a sharp increase at weaning, plateauing at adult levels by 60 days. 4. Hepatic CYP1A (mostly CYP1A2) increased sharply just before weaning. However, in contrast to the enterocytic enzyme, there was a 4-fold decrease in enzyme expression down to adult levels by day 60.

引用

页码：595 / 604

页数：10

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