Studying inner ear protein-protein interactions using FRET and FLIM

被引：17

作者：

Hallworth, Richard ^{[1
]}

Currall, Benjamin

Nichols, Michael G.

Wu, Xudong

Zuo, Jian

机构：

[1] Creighton Univ, Sch Med, Dept Biomed Sci, Omaha, NE 68178 USA

[2] Creighton Univ, Dept Phys, Omaha, NE 68178 USA

[3] St Jude Childrens Res Hosp, Dept Dev Biol, Memphis, TN 38105 USA

来源：

BRAIN RESEARCH | 2006年 / 1091卷

关键词：

hearing; cochlea; hair cell; FRET; FLIM; confocal;

D O I：

10.1016/j.brainres.2006.02.076

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Molecular genetic studies of the inner ear have recently revealed a large number of previously undescribed proteins, but their functions remain unclear. Optical methods such as FRET and FLIM are just beginning to be applied to the study of functional interactions between novel inner ear proteins. This review discusses the various methods for employing FRET and FLIM in protein-protein interaction studies, their advantages and pitfalls, with examples drawn from inner ear studies. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：122 / 131

页数：10

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