Quantitation of intracellular zidovudine phosphates by use of combined cartridge radioimmunoassay methodology

被引：45

作者：

Robbins, BL

Waibel, BH

Fridland, A

机构：

[1] ST JUDE CHILDRENS RES HOSP, DEPT INFECT DIS, MEMPHIS, TN 38105 USA

[2] UNIV TENNESSEE, DEPT PHARMACOL, MEMPHIS, TN 38101 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1996年 / 40卷 / 11期

关键词：

D O I：

10.1128/AAC.40.11.2651

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This report describes the development of a potentially clinical method to measure the cellular metabolites of zidovudine (ZDV) in patients receiving the drug, This new method combines the use of Sep-Pak cartridges to separate ZDV phosphates with radioimmunoassaying to quantitate ZDV, The detection limit is 0.02 pmol/10(6) cells, and this assay can measure a wide range of intracellular drug concentrations, The use of the cartridge-radioimmunoassay methodology should prove very useful for in vivo cellular pharmacokinetic studies of ZDV.

引用

页码：2651 / 2654

页数：4

共 23 条

[1] PHASE-1 EVALUATION OF ZIDOVUDINE ADMINISTERED TO INFANTS EXPOSED AT BIRTH TO THE HUMAN-IMMUNODEFICIENCY-VIRUS
BOUCHER, FD
MODLIN, JF
WELLER, S
RUFF, A
MIROCHNICK, M
PELTON, S
WILFERT, C
MCKINNEY, R
CRAIN, MJ
ELKINS, MM
BLUM, MR
PROBER, CG
[J]. JOURNAL OF PEDIATRICS, 1993, 122 (01) : 137 - 144
[2] THE EFFICACY OF AZIDOTHYMIDINE (AZT) IN THE TREATMENT OF PATIENTS WITH AIDS AND AIDS-RELATED COMPLEX - A DOUBLE-BLIND, PLACEBO-CONTROLLED TRIAL
FISCHL, MA
RICHMAN, DD
GRIECO, MH
GOTTLIEB, MS
VOLBERDING, PA
LASKIN, OL
LEEDOM, JM
GROOPMAN, JE
MILDVAN, D
SCHOOLEY, RT
JACKSON, GG
DURACK, DT
KING, D
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1987, 317 (04) : 185 - 191
[3] A RANDOMIZED CONTROLLED TRIAL OF A REDUCED DAILY DOSE OF ZIDOVUDINE IN PATIENTS WITH THE ACQUIRED-IMMUNODEFICIENCY-SYNDROME
FISCHL, MA
PARKER, CB
PETTINELLI, C
WULFSOHN, M
HIRSCH, MS
COLLIER, AC
ANTONISKIS, D
HO, M
RICHMAN, DD
FUCHS, E
MERIGAN, TC
REICHMAN, RC
GOLD, J
STEIGBIGEL, N
LEOUNG, GS
RASHEED, S
TSIATIS, A
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1990, 323 (15) : 1009 - 1014
[4] FRIDLAND A, 1990, MOL PHARMACOL, V37, P665
[5] PHOSPHORYLATION OF 3'-AZIDO-3'-DEOXYTHYMIDINE AND SELECTIVE INTERACTION OF THE 5'-TRIPHOSPHATE WITH HUMAN-IMMUNODEFICIENCY-VIRUS REVERSE-TRANSCRIPTASE
FURMAN, PA
FYFE, JA
STCLAIR, MH
WEINHOLD, K
RIDEOUT, JL
FREEMAN, GA
LEHRMAN, SN
BOLOGNESI, DP
BRODER, S
MITSUYA, H
BARRY, DW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) : 8333 - 8337
[6] PHASE-I/PHASE-II STUDY OF 3TC (LAMIVUDINE) IN HIV-POSITIVE, ASYMPTOMATIC OR MILD AIDS-RELATED COMPLEX PATIENTS - SUSTAINED REDUCTION IN VIRAL MARKERS
INGRAND, D
WEBER, J
BOUCHER, CAB
LOVEDAY, C
ROBERT, C
HILL, A
CAMMACK, N
KATLAMA, C
TUBIANA, R
VALENTIN, MA
GENTILINI, M
VANLEEUWEN, R
DANNER, SA
KITCHEN, V
MCBRIDE, M
[J]. AIDS, 1995, 9 (12) : 1323 - 1329
[7] KLINE MW, 1995, PEDIATRICS, V96, P247
[8] A METHOD FOR THE QUANTIFICATION OF INTRACELLULAR ZIDOVUDINE NUCLEOTIDES
KUSTER, H
VOGT, M
JOOS, B
NADAI, V
LUTHY, R
[J]. JOURNAL OF INFECTIOUS DISEASES, 1991, 164 (04) : 773 - 776
[9] 2',3'-DIDEOXYINOSINE (DDI) IN PATIENTS WITH THE ACQUIRED-IMMUNODEFICIENCY-SYNDROME OR AIDS-RELATED COMPLEX - A PHASE-I TRIAL
LAMBERT, JS
SEIDLIN, M
REICHMAN, RC
PLANK, CS
LAVERTY, M
MORSE, GD
KNUPP, C
MCLAREN, C
PETTINELLI, C
VALENTINE, FT
DOLIN, R
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1990, 322 (19) : 1333 - 1340
[10] LARDER BA, 1995, SCIENCE, V269, P969

← 1 2 3 →