Effect of sucrose on adventitious root regeneration in apple

被引:73
作者
Calamar, A
de Klerk, GJ
机构
[1] Plant Tissue Culture & Biotechnol Grp, NL-2160 AB Lisse, Netherlands
[2] Comenius Univ, Dept Plant Physiol, Bratislava 84215, Slovakia
关键词
auxin; carbohydrates; Malus; organogenesis; regeneration; rooting; tissue culture;
D O I
10.1023/A:1016356123367
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We have examined the effect of sucrose on adventitious root formation in apple microcuttings and in 1-mm stem slices cut from apple microcuttings. The sucrose concentration influenced the number of adventitious roots, but at a broad range of sucrose concentrations (1-9%) the effect was small. In addition, there was an interaction between sucrose and auxin: increasing the sucrose concentration shifted the dose-response curve of auxin to the right. When slices were cultured on medium without sucrose for the initial period (0-48 h), rooting was reduced whereas 48-h culture without application of sucrose had hardly any effect or even a slight promotive effect in a later period (48-120 h). The results show that during adventitious root formation, applied sucrose is used as a source of energy and building blocks but they are also in accordance with a possible regulatory role of sucrose.
引用
收藏
页码:207 / 212
页数:6
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