Polyethyleneimine-grafted poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible glucose oxidase immobilization

被引：62

作者：

Arica, MY ^{[1
]}

Bayramoglu, G ^{[1
]}

机构：

[1] Kirikkale Univ, Fac Sci, Biochem Proc & Biomat Res Lab, TR-71450 Kirikkale, Turkey

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2004年 / 20卷 / 01期

关键词：

membranes; immobilization; adsorption; enzyme technology; enzyme; glucose oxidase;

D O I：

10.1016/j.bej.2004.04.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glucose oxidase (GOD) was immobilized onto polyethyleneimide (PEI) grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) (poly(HEMA-co-GMA)) membranes. The maximum GOD immobilization capacities of the poly(HEMA-co-GMA-4)-PEI membrane were 84 mug/cm(2). The activities of GOD-immobilized onto poly(HEMA-co-GMA-1-5)-PEI membranes were in the range 7.6-14.5 U/cm(2). The storage conditions for the immobilized enzyme preparations were studied and the immobilized enzymes had a long-storage stability (only 7 and 24% activity decrease in 3 months under wet and dry storage conditions, respectively). (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：73 / 77

页数：5

共 16 条

[1] Reversible immobilization of urease onto Procion Brown MX-5BR-Ni(II) attached polyamide hollow-fibre membranes [J].

Akgöl, S ;

Yalçinkaya, Y ;

Bayramoglu, G ;

Denizli, A ;

Arica, MY .

PROCESS BIOCHEMISTRY, 2002, 38 (05) :675-683

[2]

[Anonymous], QUANTITATIVE ORGANIC

[3] Covalent immobilisation of invertase onto a reactive film composed of 2-hydroxyethyl methacrylate and glycidyl methacrylate:: properties and application in a continuous flow system [J].

Bayramoglu, G ;

Akgöl, S ;

Bulut, A ;

Denizli, A ;

Arica, MY .

BIOCHEMICAL ENGINEERING JOURNAL, 2003, 14 (02) :117-126

[4]

BAYRAMOGLU G, 2002, COLLOID SURF A, P41

[5] Immobilization of lipase on a polymeric microsphere with an epoxy group prepared by radiation-induced polymerization [J].

Choi, SH ;

Lee, KP ;

Kang, HD .

JOURNAL OF APPLIED POLYMER SCIENCE, 2003, 88 (05) :1153-1161

[6] Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports:: a review [J].

Durán, N ;

Rosa, MA ;

D'Annibale, A ;

Gianfreda, L .

ENZYME AND MICROBIAL TECHNOLOGY, 2002, 31 (07) :907-931

[7] Immobilization of polyphenol oxidase on chitosan-coated polysulphone capillary membranes for improved phenolic effluent bioremediation [J].

Edwards, W ;

Leukes, WD ;

Rose, PD ;

Burton, SG .

ENZYME AND MICROBIAL TECHNOLOGY, 1999, 25 (8-9) :769-773

[8] Enzymatic membrane reactors for biodegradation of recalcitrant compounds.: Application to dye decolourisation [J].

López, C ;

Mielgo, I ;

Moreira, MT ;

Feijoo, G ;

Lema, JM .

JOURNAL OF BIOTECHNOLOGY, 2002, 99 (03) :249-257

[9]

Mateo C, 2000, BIOTECHNOL BIOENG, V68, P98, DOI 10.1002/(SICI)1097-0290(20000405)68:1<98::AID-BIT12>3.0.CO

[10]

2-T

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