Radiolabeling of epidermal growth factor with Tc-99m and in vivo localization following intracerebral injection into normal and glioma-bearing rats

被引:45
作者
Capala, J
Barth, RF
Bailey, MQ
Fenstermaker, RA
Marek, MJ
Rhodes, BA
机构
[1] OHIO STATE UNIV, DEPT PATHOL, COLUMBUS, OH 43210 USA
[2] OHIO STATE UNIV, DEPT VET CLIN SCI, COLUMBUS, OH 43210 USA
[3] ROSWELL PK CANC INST, DEPT NEUROSURG, BUFFALO, NY 14273 USA
[4] RHOMED INC, ALBUQUERQUE, NM 87109 USA
关键词
D O I
10.1021/bc970031s
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
High grade gliomas may have amplified expression of the epidermal growth factor receptor (EGFR) gene c-erb-B, which often is associated with increased expression of transmembrane EGFR. The purpose of the present study was to develop a method for labeling EGF with Tc-99m and to determine whether the resulting radioligand would localize, following intracerebral injection, in rats bearing EGFR-positive gliomas. EGF has a relatively low molecular mass (similar to 6 kDa) compared to monoclonal antibodies, and this has allowed smaller bioconjugates, which should diffuse more rapidly within the brain and more effectively target disseminated glioma cells, to be constructed. In the present study, EGF has been labeled with either I-131 or (TC)-T-99m, and in vitro uptake of the resulting radioligand has been investigated using C6(EGFR) rat glioma cells, which had been transfected with the EGFR gene. Cellular uptake of I-131 radioactivity peaked after similar to 30 min of incubation with [I-131]EGF, following which time it declined, while Tc-99m radioactivity continued to increase over a 6 h incubation with [Tc-99m]-EGF. To determine if radiolabeled EGF had in vivo tumor-localizing properties, C6(EGFR) glioma cells were implanted stereotactically into the brains of Fischer rats. Four weeks later, either Tc-99m- or I-131-labeled EGF was injected intracerebrally into normal or glioma-bearing animals using the same stereotactic coordinates. External gamma scintigraphy revealed that I-131 radioactivity disappeared rapidly from the brain regions of tumor-bearing animals compared to Tc-99m, similar to 50% of which remained in the tumor for up to 12 h. In contrast, only similar to 20% remained in the brains of non-tumor-bearing animals after 6 h. These studies are the first to describe a method for radiolabeling EGF with Tc-99m and to detect it by external scintigraphy in the brains of tumor-bearing animals.
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收藏
页码:289 / 295
页数:7
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